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Document Type |
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Article In Journal |
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Document Title |
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Anti-inflammatory role of sesamin in STZ induced mice model of diabetic retinopathy Anti-inflammatory role of sesamin in STZ induced mice model of diabetic retinopathy |
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Document Language |
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English |
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Abstract |
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Diabetic retinopathy (DR) is the common cause of diabetic vascular complications that leads to the blindness in the
working age population throughout the world. Free radicals mediated oxidative stress and inflammation play a
significant role in pathophysiology of DR. To find a new and safe drug to treat DR is still challenging and for that
purpose the natural compounds may be therapeutic agents. Here we show that sesamin (SES), which is the main
component of sesame seed and its oil, and has been reported as potent antioxidant and neuroprotective, could be
a therapeutic agent in DR. In the present study, we investigated protective effect of SES in Streptozotocin (STZ) induced
DR in mice. The mice were divided into three groups (Control, DR and DR+SES) for the study. After two
weeks post-diabetic establishment, mice were treated with SES (30 mg/kg BW, i.p, alternate day) for four weeks.
Mice body weight and blood glucose level were measured from each group. The microglial activation of retina
was determined by immunohistochemistry analysis by using Iba-1 as a microglia marker. Retinal mRNA levels of
Iba-1, tumor necrosis factor-α (TNF-α), inducible nitric oxide synthase (iNOS) and Intercellular Adhesion
Molecule 1 (ICAM-1) were examined by qRT-PCR. The level of iNOS protein expression was examined by
immunoblotting. Together these data demonstrate that SES treatment lowered the progression of diabetic retinal injury
by: 1) decreasing blood glucose level, 2) suppressing microglia activation, 3) reducing retinal TNF-α and ICAM-
1 levels and 4) quenching iNOS expression. In conclusion, the results suggest that SES treatment may be of therapeutic
benefit in reducing the progression of DR by ameliorating hyperglycemia and inflammation in diabetic retina. |
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ISSN |
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0165-5728 |
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Journal Name |
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Journal of neuroimmunology |
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Volume |
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295 |
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Issue Number |
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1 |
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Publishing Year |
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1437 AH
2016 AD |
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Article Type |
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Article |
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Added Date |
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Wednesday, July 12, 2017 |
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Researchers
| Saif Ahmad | Ahmad, Saif | Researcher | Doctorate | |
| Nehal M. ElSherbiny | ElSherbiny, Nehal M. | Researcher | Doctorate | |
| Mohammad Sarwar Jamal | Jamal, Mohammad Sarwar | Researcher | Doctorate | |
| Faisal A. Alzahrani | Alzahrani, Faisal A. | Researcher | Doctorate | |
| Rizwanul Haque | Haque, Rizwanul | Researcher | Doctorate | |
| Raziuddin Khan | Khan, Raziuddin | Researcher | Doctorate | |
| Syed Kashif Zaidi | Zaidi, Syed Kashif | Researcher | Doctorate | |
| Mohammed H. AlQahtani | AlQahtani, Mohammed H. | Researcher | Doctorate | |
| Gregory I. Liou | Liou, Gregory I. | Researcher | Doctorate | |
| Kanchan Bhatia | Bhatia, Kanchan | Researcher | Doctorate | |
|