Molecular Service Unit
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Molecular Service Unit |
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Molecular Service Unit
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Coordinator:
Prof Mohammed Al-Qahtani
Consultant (1):
Prof Adel Abuzenadah
Consultant (2):
Prof Adeel Chaudhary
The Molecular Service Unit constitutes the core of CEGMR research and training, which has a number of full-time support personnel that provide DNA sequencing and molecular biology training for the whole Department.
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Services:
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DNA and RNA extractions |
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Pre-and post-PCR preparation |
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Gel electrophoresis and documentation |
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Restriction Enzyme Fragment Analysis |
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New generation capillary electrophoresis for protein separation |
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Student training using multi-objective microscope |
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Real-time PCR |
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Sequencing and GeneScan |
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FISH preparation |
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Immuno-histochemistry preparation |
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Sequencing services:
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Sanger Sequencing |
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Next Generation sequencing |
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Genotyping |
Gene expression sequencing:
Platforms
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Affymetrix platform (Gene expression profiling, targeted re-sequencing) |
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Agilent platform (Array CGH and SNP analysis, Gene expression profiling, miRNA profiling) |
Mass spectrometry and Nano UPLC
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Protein separation and identification using MS/MS platform |
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Nano LC MS/MS and MALDI TOF/TOF |
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Nano scale protein purification and identification |
Real-Time PCR based validation services
Equipments Available:
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97500-series tables "UV trans illuminator" (Cole Parmer). |
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Centrifuge 5810R (Eppendorf). |
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Chemical Fume Hood (Hamilton). |
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CX Series Ultraviolet - Fluorescence Analysis Cabinet (Speetroline). |
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DNA Sequencing System "conformational sensitive gel electrophoresis unit" (ThermoEC). |
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Frost free commercial freezer (Frigidaire). |
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Gel documentation system (BioRad). |
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Gel documentation system (UVitec). |
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ISS110 Speed Vac System (Thermo Savant). |
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No frost freezer (Whirlpool). |
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No frost freezer "2 freezers" (DORA). |
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Olympus BX51 "multiuser microscope" (Olympus Optical Co., LTD). |
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Refrigerator (Kelvinator Platinum). |
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Shaking water bath (SHEL LAB). |
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Sterol/ GARD Hood CLASS II Type A/ B3 "Safety cabinet" (The Baker Company). |
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Water PRO PS "Water purification system" (LABCONCO). |
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Last Update
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3/1/2015 1:17:45 PM
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