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Document Type |
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Article In Journal |
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Document Title |
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Candida identification: a journey from conventional to molecular methods in medical mycology. Candida identification: a journey from conventional to molecular methods in medical mycology. |
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Document Language |
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English |
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Abstract |
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The incidence of Candida infections have increased substantially in recent years due to aggressive use of immunosuppressants among patients. Use of broad-spectrum antibiotics and intravascular catheters in the intensive care unit have also attributed with high risks of candidiasis among immunocompromised patients. Among Candida species, C. albicans accounts for the majority of superficial and systemic infections, usually associated with high morbidity and mortality often caused due to increase in antimicrobial resistance and restricted number of antifungal drugs. Therefore, early detection of candidemia and correct identification of Candida species are indispensable pre-requisites for appropriate therapeutic intervention. Since blood culture based methods lack sensitivity, and species-specific identification by conventional method is time-consuming and often leads to misdiagnosis within closely related species, hence, molecular methods may provide alternative for accurate and rapid identification of Candida species. Although, several molecular approaches have been developed for accurate identification of Candida species but the internal transcribed spacer 1 and 2 (ITS1 and ITS2) regions of the rRNA gene are being used extensively in a variety of formats. Of note, ITS sequencing and PCR-RFLP analysis of ITS region seems to be promising as a rapid, easy, and cost-effective method for identification of Candida species. Here, we review a number of existing techniques ranging from conventional to molecular approaches currently in use for the identification of Candida species. Further, advantages and limitations of these methods are also discussed with respect to their discriminatory power, reproducibility, and ease of performance |
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ISSN |
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0959-3993 |
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Journal Name |
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World Journal of Microbiology and Biotechnology |
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Volume |
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30 |
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Issue Number |
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5 |
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Publishing Year |
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1435 AH
2014 AD |
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Article Type |
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Article |
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Added Date |
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Thursday, March 10, 2016 |
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Researchers
| Mohammad Zubai Alam | Alam, Mohammad Zubai | Investigator | | |
| Qamre Alam | Alam, Qamre | Researcher | | |
| Asif Jiman-Fatani | Jiman-Fatani, Asif | Researcher | | |
| Mohammad Amjad Kamal | Kamal, Mohammad Amjad | Researcher | | |
| Adel M Abuzenadah | Abuzenadah, Adel M | Researcher | | |
| Adeel G Chaudhary | Chaudhary, Adeel G | Researcher | | |
| Mohammad Akram | Akram, Mohammad | Researcher | | |
| Absarul Haque | Haque, Absarul | Researcher | | |
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