Document Details

Document Type : Article In Journal 
Document Title :
A technique for setting analytical thresholds in massively parallel sequencing-based forensic DNA analysis
A technique for setting analytical thresholds in massively parallel sequencing-based forensic DNA analysis
 
Document Language : English 
Abstract : Amplicon (targeted) sequencing by massively parallel sequencing (PCR-MPS) is a potential method for use in forensic DNA analyses. In this application, PCR-MPS may supplement or replace other instrumental analysis methods such as capillary electrophoresis and Sanger sequencing for STR and mitochondrial DNA typing, respectively. PCR-MPS also may enable the expansion of forensic DNA analysis methods to include new marker systems such as single nucleotide polymorphisms (SNPs) and insertion/deletions (indels) that currently are assayable using various instrumental analysis methods including microarray and quantitative PCR. Acceptance of PCR-MPS as a forensic method will depend in part upon developing protocols and criteria that define the limitations of a method, including a defensible analytical threshold or method detection limit. This paper describes an approach to establish objective analytical thresholds suitable for multiplexed PCR-MPS methods. A definition is proposed for PCR-MPS method background noise, and an analytical threshold based on background noise is described. 
ISSN : 1932-6203 
Journal Name : PLOS ONE 
Volume : 12 
Issue Number : 5 
Publishing Year : 1438 AH
2017 AD 
Article Type : Article 
Added Date : Wednesday, June 26, 2019 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
Brian YoungYoung, Brian ResearcherDoctoratebrian@nichevision.com
Jonathan L. KingKing, Jonathan L.ResearcherDoctorate 
Bruce BudowleBudowle, Bruce ResearcherDoctorate 
Luigi ArmogidaArmogida, Luigi ResearcherDoctorate 

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