Document Details

Document Type : Article In Journal 
Document Title :
Direct PCR amplification of DNA from human bloodstains, saliva, and touch samples collected with microFLOQ® swabs
Direct PCR amplification of DNA from human bloodstains, saliva, and touch samples collected with microFLOQ® swabs
Document Language : English 
Abstract : Previous studies have shown that nylon flocked swabs outperform traditional fiber swabs in DNA recovery due to their innovative design and lack of internal absorbent core to entrap cellular materials. The microFLOQ® Direct swab, a miniaturized version of the 4N6 FLOQSwab®, has a small swab head that is treated with a lysing agent which allows for direct amplification and DNA profiling from sample collection to final result in less than two hours. Additionally, the microFLOQ® system subsamples only a minute portion of a stain and preserves the vast majority of the sample for subsequent testing or re-analysis, if desired. The efficacy of direct amplification of DNA from dilute bloodstains, saliva stains, and touch samples was evaluated using microFLOQ® Direct swabs and the GlobalFiler™ Express system. Comparisons were made to traditional methods to assess the robustness of this alternate workflow. Controlled studies with 1:19 and 1:99 dilutions of bloodstains and saliva stains consistently yielded higher STR peak heights than standard methods with 1ng input DNA from the same samples. Touch samples from common items yielded single source and mixed profiles that were consistent with primary users of the objects. With this novel methodology/workflow, no sample loss occurs and therefore more template DNA is available during amplification. This approach may have important implications for analysis of low quantity and/or degraded samples that plague forensic casework. 
ISSN : 1872-4973 
Journal Name : Forensic Science International: Genetics 
Volume : 32 
Issue Number : 1 
Publishing Year : 1439 AH
2018 AD
Article Type : Article 
Added Date : Monday, June 24, 2019 


Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
Angie AmbersAmbers, Angie
Rachel WileyWiley, Rachel ResearcherDoctorate 
Nicole NovroskiNovroski, Nicole ResearcherDoctorate 
Bruce BudowleBudowle, Bruce ResearcherDoctorate 


File NameTypeDescription
 44526.pdf pdf 

Back To Researches Page