Document Details

Document Type : Article In Journal 
Document Title :
Effects of the Ion PGM™ Hi-Q™ sequencing chemistry on sequence data quality
Effects of the Ion PGM™ Hi-Q™ sequencing chemistry on sequence data quality
 
Document Language : English 
Abstract : Massively parallel sequencing (MPS) offers substantial improvements over current forensic DNA typing methodologies such as increased resolution, scalability, and throughput. The Ion PGM™ is a promising MPS platform for analysis of forensic biological evidence. The system employs a sequencing-by-synthesis chemistry on a semiconductor chip that measures a pH change due to the release of hydrogen ions as nucleotides are incorporated into the growing DNA strands. However, implementation of MPS into forensic laboratories requires a robust chemistry. Ion Torrent’s Hi-Q™ Sequencing Chemistry was evaluated to determine if it could improve on the quality of the generated sequence data in association with selected genetic marker targets. The whole mitochondrial genome and the HID-Ion STR 10-plex panel were sequenced on the Ion PGM™ system with the Ion PGM™ Sequencing 400 Kit and the Ion PGM™ Hi-Q™ Sequencing Kit. Concordance, coverage, strand balance, noise, and deletion ratios were assessed in evaluating the performance of the Ion PGM™Hi-Q™Sequencing Kit. The results indicate that reliable, accurate data are generated and that sequencing through homopolymeric regions can be improved with the use of Ion Torrent’s Hi-Q™ Sequencing Chemistry. Overall, the quality of the generated sequencing data supports the potential for use of the Ion PGM™ in forensic genetic laboratories. 
ISSN : 0937-9827 
Journal Name : International journal of legal medicine 
Volume : 130 
Issue Number : 5 
Publishing Year : 1437 AH
2016 AD 
Article Type : Article 
Added Date : Tuesday, July 11, 2017 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
Jennifer D. ChurchillChurchill, Jennifer D.ResearcherDoctorate 
Jonathan L. KingKing, Jonathan L.ResearcherDoctorate 
Ranajit ChakrabortyChakraborty, Ranajit ResearcherDoctorate 
Bruce BudowleBudowle, Bruce ResearcherDoctorate 

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