Document Details

Document Type : Article In Journal 
Document Title :
Pelleted bone marrow derived mesenchymal stem cells are better protected from the deleterious effects of arthroscopic heat shock
Pelleted bone marrow derived mesenchymal stem cells are better protected from the deleterious effects of arthroscopic heat shock
 
Document Language : English 
Abstract : Introduction:Theimpactofarthroscopictemperatureonjointtissuesispoorlyunderstoodanditisnotknownhowmesenchymalstemcells(MSCs)respondtotheeffectsofheatgeneratedbythedeviceduringtheprocessofarthroscopyassistedexperimentalcell-basedtherapy.Inthepresentstudy,weisolatedandphenotypicallycharacterizedhumanbonemarrowmesenchymalstemcells(hBMMSCs)fromosteoarthritis(OA)patients,andevaluatedtheeffectofarthroscopicheatoncellsinsuspensionandpelletcultures. Methods:PrimaryculturesofhBMMSCswereisolatedfrombonemarrowaspiratesofOApatientsandculturedusingDMEMsupplementedwith10%FBSandcharacterizedfortheirstemness.hBMMSCs(16×10cells)culturedassinglecellsuspensionsorcellpelletswereexposedtoanilluminatedarthroscopefor10,20,or30min.Thiswasfollowedbyanalysisofcellularproliferationandheatshockrelatedgeneexpression. Results:hBMMSCswereviableandexhibitedpopulationdoubling,shortspindlemorphology,MSCrelatedCDsurfacemarkersexpressionandtri-lineagedifferentiationintoadipocytes,chondrocytesandosteoblasts.Chondrogenicandosteogenicdifferentiationincreasedcollagenproductionandalkalinephosphataseactivity.ExposureofhBMMSCstoanilluminatedarthroscopefor10,20,or30minfor72hdecreasedmetabolicactivityofthecellsinsuspensions(63.27%at30min)andincreasedmetabolicactivityincellpellets(62.86%at10minand68.57%at20min).hBMMSCsexposedto37,45,and55◦Cfor120sdemonstratedsignificantupregulationofBAX,P53,CyclinA2,CyclinE1,TNF-a,andHSP70incellsuspensionscomparedtocellpellets. Conclusions:hBMMSCcellpelletsarebetterprotectedfromtemperaturealterationscomparedtocellsuspensions.TransplantationofhBMMSCsaspelletsratherthanascellsuspensionstothecartilagedefectsitewouldthereforesupporttheirviabilityandmayaidenhancedcartilageregeneration. 
ISSN : 103-389 
Journal Name : Frontiers in physiology 
Volume : 7 
Issue Number : 1 
Publishing Year : 1437 AH
2016 AD 
Article Type : Article 
Added Date : Sunday, July 9, 2017 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
Gauthaman KalamegamKalamegam, Gauthaman ResearcherDoctorate 
Mohammed AbbasAbbas, Mohammed InvestigatorDoctorate 
Mohammed AlkaffAlkaff, Mohammed ResearcherDoctorate 
Mamdooh GariGari, Mamdooh ResearcherDoctorate 
Ali MobasheriMobasheri, Ali ResearcherDoctorate 
Adel AbuzenadahAbuzenadah, Adel ResearcherDoctorate 
Haneen AlsehliAlsehli, Haneen ResearcherDoctorate 
Roaa KadamKadam, Roaa ResearcherDoctorate 
Adeel ChaudharyChaudhary, Adeel ResearcherDoctorate 
Mohammed Al-QahtaniAl-Qahtani, Mohammed ResearcherDoctorate 
Wael KafienahKafienah, Wael ResearcherDoctorate 

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