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Document Type |
: |
Article In Journal |
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Document Title |
: |
Genotoxicity and immunotoxic effects of 1, 2-dichloroethane in Wistar rats Genotoxicity and immunotoxic effects of 1, 2-dichloroethane in Wistar rats |
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Document Language |
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English |
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Abstract |
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Dichloroethane is widely used as a solvent, degreasing agent and
in a variety of commercial products, and is known for being a ubiquitous
contaminant in the environment. Important sources principally
include the emissions from industrial processes, improper
consumption, storage, and disposal methods. In view of the fact
that the mechanism of its genotoxicity has not been satisfactorily
elucidated, the acute in vivo toxicological impact is assessed
in Rattus norvegicus. A systematic investigation has been made
involving the use of conventional methods along with molecular
and flow cytometric approaches. The micronucleus and chromosomal
aberration frequencies were significantly elevated in
bone marrow cells exposed to three concentrations at multiple
treatment durations indicating positive time- and dose-response
relationships. The mitotic index significantly decreased in similar
concentrations in contrast to normal control. Separate studies
were performed on blood cells for comet assay. It revealed
dichloroethane-induced DNA damage in all exposures readily
explainable in a dose- and time-dependent manner. Recent
molecular techniqueswere further employed using leukocytes for
the cell apoptosis/cycle and mitochondrial membrane potential
employing propidium iodide staining and rhodamine-123, respectively.
The effect on mitochondrial membrane permeability, cell
cycle phases, and the DNA damage was analyzed through flow
cytometry. These indicators revealed dichloroethane treatment
decreased the mitochondrial membrane potential, affected the
cell cycle, and confirmed the DNA damage, leading to apoptosis
of the cells of the immune system responsible for immunotoxic
effects of dichloroethane on rat leukocytes. |
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ISSN |
: |
1059-0501 |
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Journal Name |
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Journal of Environmental Science and Health, Part C |
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Volume |
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34 |
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Issue Number |
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3 |
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Publishing Year |
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1437 AH
2016 AD |
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Article Type |
: |
Article |
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Added Date |
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Sunday, July 9, 2017 |
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Researchers
| Mohammad Iqbal Lone | Lone, Mohammad Iqbal | Investigator | Doctorate | |
| Nazia Nazam | Nazam, Nazia | Researcher | Doctorate | |
| Aashiq Hussain | Hussain, Aashiq | Researcher | Doctorate | |
| Shashank K Singh | Singh, Shashank K | Researcher | Doctorate | |
| Abid Hamid Dar | Dar, Abid Hamid | Researcher | Doctorate | |
| Rauf Ahmad Najar | Najar, Rauf Ahmad | Researcher | Doctorate | |
| Mohammed Hussein Al-Qahtani | Al-Qahtani, Mohammed Hussein | Researcher | Doctorate | |
| Waseem Ahmad | Ahmad, Waseem | Researcher | Doctorate | |
|