Document Details

Document Type : Article In Journal 
Document Title :
Evaluation of the Qiagen 140-SNP forensic identification multiplex for massively parallel sequencing
Evaluation of the Qiagen 140-SNP forensic identification multiplex for massively parallel sequencing
 
Document Language : English 
Abstract : A new forensic 140-SNP genotyping system from Qiagen, designed for massively parallel sequencing (MPS) analysis, was evaluated using the Ion PGMTM MPS system. Assessments consisted of the sequencing of: established control DNAs that had been previously genotyped with alternative PCR and library preparation kits supplied by Thermo Fisher Scientific for the Ion PGMTM system; a simple set of artificial DNA mixtures; DNA extracted from a degraded femur; and a dilution series to gauge forensic sensitivity. In addition to the reagents for the DNA target capture PCR and library preparation, Qiagen offer an alternative sequence analysis software system (Workbench), which was assessed alongside the Ion PGMTM Genotyper software for forensic MPS analysis. The Qiagen SNP genotyping system produced full genotyping concordance with previous data obtained with a similar SNP panel on the Ion PGMTM and in comparison to genotypes listed for 139 of the 140 SNPs in 1000 Genomes. The workbench software was as reliable as Genotyper in calling genotypes, although scrutiny of sequence data with IGV revealed the problem of sequence misalignment plagues a small proportion of the 140 SNPs in the Qiagen panel, a problem already recognized in multiple MPS studies of the same markers in alternative kits. The potential for genotype miscalls from sequence misalignment in certain SNPs will require manual inspection in cases where low-level or degraded DNA reduces the sequence coverage to a point where misalignment influences individual SNP genotype quality 
ISSN : 1872-4973 
Journal Name : Forensic Science International: Genetics 
Volume : 28 
Issue Number : 1 
Publishing Year : 1438 AH
2017 AD 
Article Type : Article 
Added Date : Tuesday, May 16, 2017 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
M de la Puentede la Puente, M InvestigatorDoctorate 
C PhillipsPhillips, C ResearcherDoctorate 
C SantosSantos, C ResearcherDoctorate 
M FondevilaFondevila, M ResearcherDoctorate 
Á CarracedoCarracedo, Á ResearcherDoctorate 
M V LareuLareu, M VResearcher  

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