Document Details

Document Type : Article In Journal 
Document Title :
Single-strand DNA-binding protein SSB1 facilitates TERT recruitment to telomeres and maintains telomere G-overhangs
Single-strand DNA-binding protein SSB1 facilitates TERT recruitment to telomeres and maintains telomere G-overhangs
 
Document Language : English 
Abstract : Proliferating mammalian stem and cancer cells express telomerase [telomerase reverse transcriptase (TERT)] in an effort to extend chromosomal G-overhangs and maintain telomere ends. Telomerase-expressing cells also have higher levels of the single-stranded DNA-binding protein SSB1, which has a critical role in DNA double-strand break (DSB) repair. Here, we report that SSB1 binds specifically to G-strand telomeric DNA in vitro and associates with telomeres in vivo. SSB1 interacts with the TERT catalytic subunit and regulates its interaction with telomeres. Deletion of SSB1 reduces TERT interaction with telomeres and leads to G-overhang loss. Although SSB1 is recruited to DSB sites, we found no corresponding change in TERT levels at these sites, implying that SSB1-TERT interaction relies upon a specific chromatin structure or context. Our findings offer an explanation for how telomerase is recruited to telomeres to facilitate G-strand DNA extension, a critical step in maintaining telomere ends and cell viability in all cancer cells. Cancer Res; 75(5); 858-69. ©2015 AACR. 
ISSN : 1538-7445 
Journal Name : Cancer Res 
Volume : 75 
Issue Number : 5 
Publishing Year : 1436 AH
2015 AD 
Article Type : Article 
Added Date : Tuesday, April 26, 2016 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
Raj K. PanditaPandita, Raj K.Investigator  
Tracy T. ChowChow, Tracy T.Researcher  
Durga UdayakumarUdayakumar, Durga Researcher  
Amanda L. BainBain, Amanda L.Researcher  
Liza CubedduCubeddu, Liza Researcher  
Clayton R. HuntHunt, Clayton R.Researcher  
Wei ShiShi, Wei Researcher  
Nobuo HorikoshiHorikoshi, Nobuo Researcher  
Yong ZhaoZhao, Yong Researcher  
Woodring E. WrightWright, Woodring E.Researcher  
Kum Kum KhannaKhanna, Kum KumResearcher  
Jerry W. ShayShay, Jerry W.Researcher jerry.shay@utsouthwestern.edu
Tej K. PanditaPandita, Tej K.Researcher tpandita@houstonmethodist.org

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