Document Details

Document Type : Article In Journal 
Document Title :
Biased allelic expression in human primary fibroblast single cells
Biased allelic expression in human primary fibroblast single cells
 
Document Language : English 
Abstract : The study of gene expression in mammalian single cells via genomic technologies now provides the possibility to investigate the patterns of allelic gene expression. We used single-cell RNA sequencing to detect the allele-specific mRNA level in 203 single human primary fibroblasts over 133,633 unique heterozygous single-nucleotide variants (hetSNVs). We observed that at the snapshot of analyses, each cell contained mostly transcripts from one allele from the majority of genes; indeed, 76.4% of the hetSNVs displayed stochastic monoallelic expression in single cells. Remarkably, adjacent hetSNVs exhibited a haplotype-consistent allelic ratio; in contrast, distant sites located in two different genes were independent of the haplotype structure. Moreover, the allele-specific expression in single cells correlated with the abundance of the cellular transcript. We observed that genes expressing both alleles in the majority of the single cells at a given time point were rare and enriched with highly expressed genes. The relative abundance of each allele in a cell was controlled by some regulatory mechanisms given that we observed related single-cell allelic profiles according to genes. Overall, these results have direct implications in cellular phenotypic variability. 
ISSN : 1537-6605 
Journal Name : Am J Hum Genet 
Volume : 96 
Issue Number : 1 
Publishing Year : 1436 AH
2015 AD 
Article Type : Article 
Added Date : Monday, April 18, 2016 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
Christelle BorelBorel, Christelle Investigator  
Pedro G. FerreiraFerreira, Pedro G.Researcher  
Federico SantoniSantoni, Federico Researcher  
Olivier DelaneauDelaneau, Olivier Researcher  
Alexandre FortFort, Alexandre Researcher  
Konstantin Y. PopadinPopadin, Konstantin Y.Researcher  
Marco GarieriGarieri, Marco Researcher  
Emilie EmilieEmilie, Emilie Researcher  
Pascale RibauxRibaux, Pascale Researcher  
Michel GuipponiGuipponi, Michel Researcher  
Ismael PadioleauPadioleau, Ismael Researcher  
Piero CarninciCarninci, Piero Researcher  
Emmanouil T. DermitzakisDermitzakis, Emmanouil T.Researcher emmanouil.dermitzakis@unige.ch
Stylianos E. AntonarakisAntonarakis, Stylianos E.Researcher stylianos.antonarakis@unige.ch

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