Document Details

Document Type : Article In Journal 
Document Title :
Functional epigenetic approach identifies frequently methylated genes in Ewing sarcoma
Functional epigenetic approach identifies frequently methylated genes in Ewing sarcoma
 
Document Language : English 
Abstract : Using a candidate gene approach we recently identified frequent methylation of the RASSF2 gene associated with poor overall survival in Ewing sarcoma (ES). To identify effective biomarkers in ES on a genome-wide scale, we used a functionally proven epigenetic approach, in which gene expression was induced in ES cell lines by treatment with a demethylating agent followed by hybridization onto high density gene expression microarrays. After following a strict selection criterion, 34 genes were selected for expression and methylation analysis in ES cell lines and primary ES. Eight genes (CTHRC1, DNAJA4, ECHDC2, NEFH, NPTX2, PHF11, RARRES2, TSGA14) showed methylation frequencies of>20% in ES tumors (range 24-71%), these genes were expressed in human bone marrow derived mesenchymal stem cells (hBMSC) and hypermethylation was associated with transcriptional silencing. Methylation of NPTX2 or PHF11 was associated with poorer prognosis in ES. In addition, six of the above genes also showed methylation frequency of>20% (range 36-50%) in osteosarcomas. Identification of these genes may provide insights into bone cancer tumorigenesis and development of epigenetic biomarkers for prognosis and detection of these rare tumor types. 
ISSN : 1559-2294 
Journal Name : Epigenetics 
Volume : 8 
Issue Number : 11 
Publishing Year : 1434 AH
2013 AD 
Article Type : Article 
Added Date : Thursday, April 14, 2016 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
Abdullah AlholleAlholle, Abdullah Researcher  
Anna T BriniBrini, Anna T Researcher  
Seley GharaneiGharanei, Seley Researcher  
Sumathi VaiyapuriVaiyapuri, Sumathi Researcher  
Elena ArrigoniArrigoni, Elena Researcher  
Ashraf DallolDallol, Ashraf Researcher  
Dean GentleGentle, Dean Researcher  
Takeshi KishidaKishida, Takeshi Researcher  
Toru HirumaHiruma, Toru Researcher  
Farida LatifLatif, Farida Investigator  

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