Document Details

Document Type : Article In Journal 
Document Title :
RASSF2 methylation is a strong prognostic marker in younger age patients with Ewing sarcoma
RASSF2 methylation is a strong prognostic marker in younger age patients with Ewing sarcoma
 
Document Language : English 
Abstract : Ras-association domain family of genes consist of 10 members (RASSF1-RASSF10), all containing a Ras-association (RA) domain in either the C- or the N-terminus. Several members of this gene family are frequently methylated in common sporadic cancers; however, the role of the RASSF gene family in rare types of cancers, such as bone cancer, has remained largely uninvestigated. In this report, we investigated the methylation status of RASSF1A and RASSF2 in Ewing sarcoma (ES). Quantitative real-time methylation analysis (MethyLight) demonstrated that both genes were frequently methylated in Ewing sarcoma tumors (52.5% and 42.5%, respectively) as well as in ES cell lines and gene expression was upregulated in methylated cell lines after treatment with 5-aza-2'-deoxcytidine. Overexpression of either RASSF1A or RASSF2 reduced colony formation ability of ES cells. RASSF2 methylation correlated with poor overall survival (p = 0.028) and this association was more pronounced in patients under the age of 18 y (p = 0.002). These results suggest that both RASSF1A and RASSF2 are novel epigenetically inactivated tumor suppressor genes in Ewing sarcoma and RASSF2 methylation may have prognostic implications for ES patients. 
ISSN : 1559-2294 
Journal Name : Epigenetics 
Volume : 8 
Issue Number : 9 
Publishing Year : 1434 AH
2013 AD 
Article Type : Article 
Added Date : Wednesday, April 6, 2016 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
Seley GharaneiGharanei, Seley Investigator  
Anna T BriniBrini, Anna T Researcher  
Sumathi VaiyapuriVaiyapuri, Sumathi Researcher  
Abdullah AlholleAlholle, Abdullah Researcher  
Ashraf DallolDallol, Ashraf Researcher  
Elena ArrigoniArrigoni, Elena Researcher  
Takeshi KishidaKishida, Takeshi Researcher  

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