Document Details

Document Type : Article In Journal 
Document Title :
Decreased GABABR expression and increased neuronal cell death in developing rat brain after PTZ-induced seizure
Decreased GABABR expression and increased neuronal cell death in developing rat brain after PTZ-induced seizure
 
Document Language : English 
Abstract : The objective of this study was to evaluate the PTZ-induced seizures effects on GABAB receptor (R) expression and to observe its neurodegenerative effect in hippocampal part of developing rat brain. In the present study, high dose of pentylenetetrazol (PTZ 40 mg/kg) was injected in developing rats of age 5 weeks having average weight of 60-65 g for 4 days. Further, baclofen (B 3 mg/kg i.p) agonist and phaclofen (P 30 μg/rat) antagonist of GABABR were injected along with PTZ. Western blot analysis was used to elucidate expression of GABABR protein upon PTZ, baclofen and phaclofen exposure in the developing rat brain. Furthermore, PTZ-induced apoptotic neurodegeneration was also observed through the release of caspase-3 antibody and propidium iodide (PI) staining using confocal microscopy. Seizure was confirmed using electroencephalography (EEG) data obtained from the Laxtha EEG-monitoring device in the EEG recording room and EEG was monitored 5-15 min after PTZ injection. The results of the present study showed that PTZ-induced seizure significantly decreased GABABR expression and induced neuronal apoptosis in cortical and hippocampal part of brain. While, baclofen reverse the effect of PTZ by increasing the expression of GABABR as compared to the PTZ- , PTZ plus B- and PTZ plus P-treated groups. Our findings indicated that PTZ-induced seizure showed not only decrease in GABABR expression but also cause neuronal apoptosis in the developing rat brain. 
ISSN : 1590-1874 
Journal Name : Neurological Sciences 
Volume : 34 
Issue Number : 4 
Publishing Year : 1434 AH
2013 AD 
Article Type : Article 
Added Date : Tuesday, March 15, 2016 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
Muhammad Imran NaseerNaseer, Muhammad Imran Investigator  
Ikram UllahUllah, Ikram Researcher  
Mohammed H Al-QahtaniAl-Qahtani, Mohammed H Researcher  
Sajjad KarimKarim, Sajjad Researcher  
Najeeb UllahUllah, Najeeb Researcher  
Shakeel Ahmed AnsariAnsari, Shakeel Ahmed Researcher  
Myeong Ok KimKim, Myeong Ok Researcher  
Fehmida BibiBibi, Fehmida Researcher  

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