Document Details

Document Type : Article In Journal 
Document Title :
Interleukin-33 amplifies IgE synthesis and triggers mast cell degranulation via interleukin-4 in naïve mice
Interleukin-33 amplifies IgE synthesis and triggers mast cell degranulation via interleukin-4 in naïve mice
 
Document Language : English 
Abstract : BACKGROUND: The regulation and function of IgE in healthy individuals and in antigen-naïve animals is not well understood. IL-33 administration increases serum IgE in mice with unknown mechanism. We tested the hypothesis that IL-33 provides an antigen-independent stimulus for IgE production and mast cell degranulation. METHODS: IL-33 was administered to naïve wild-type (WT), nude and ST2(-/-) , IL-4(-/-) , IL4Rα(-/-) and T-or B-cell-specific IL-4Rα(-/-) mice. IgE and cytokines were quantified by ELISA. T- and B-lymphocyte numbers and CD40L expression were determined by flow cytometry. Anaphylaxis was measured by temperature, mast cell degranulation and histamine release. RESULTS: IL-33 enhanced IgE production in naïve WT, T-IL-4Rα(-/-) but not in ST2(-/-) , IL-4(-/-) , IL-4Rα(-/-) or B-cell-specific IL-4Rα(-/-) mice, demonstrating IL-33 specificity and IL-4 dependency. Moreover, IL-4 was required for IL-33-induced B-cell proliferation and T-cell CD40L expression, which promotes IgE production. IL-33-induced IL-4 production was mainly from innate cells including mast cells and eosinophils. IL-33 increased mast cell surface IgE and triggered degranulation and systemic anaphylaxis in allergen-naïve WT but not in IL-4Rα(-/-) mice. CONCLUSION: IL-33 amplifies IgE synthesis and triggers anaphylaxis in naïve mice via IL-4, independent of allergen. IL-33 may play an important role in nonatopic allergy and idiopathic anaphylaxis. 
ISSN : 1398-9995 
Journal Name : Allergy 
Volume : 67 
Issue Number : 9 
Publishing Year : 1433 AH
2012 AD 
Article Type : Article 
Added Date : Thursday, March 10, 2016 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
M Komai‐KomaKomai‐Koma, M Investigator  
F BrombacherBrombacher, F Researcher  
PN PushparajPushparaj, PN Researcher  
B ArendseArendse, B Researcher  
C McSharryMcSharry, C Researcher  
J AlexanderAlexander, J Researcher  
R ChaudhuriChaudhuri, R Researcher  
NC ThomsonThomson, NC Researcher  
ANJ McKenzieMcKenzie, ANJ Researcher  
I McInnesMcInnes, I Researcher  

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