Document Details

Document Type : Article In Journal 
Document Title :
Transcriptome analysis of amoeboid and ramified microglia isolated from the corpus callosum of rat brain
Transcriptome analysis of amoeboid and ramified microglia isolated from the corpus callosum of rat brain
 
Document Language : English 
Abstract : BACKGROUND: Microglia, the resident immune cells of the central nervous system (CNS), have two distinct phenotypes in the developing brain: amoeboid form, known to be amoeboid microglial cells (AMC) and ramified form, known to be ramified microglial cells (RMC). The AMC are characterized by being proliferative, phagocytic and migratory whereas the RMC are quiescent and exhibit a slow turnover rate. The AMC transform into RMC with advancing age, and this transformation is indicative of the gradual shift in the microglial functions. Both AMC and RMC respond to CNS inflammation, and they become hypertrophic when activated by trauma, infection or neurodegenerative stimuli. The molecular mechanisms and functional significance of morphological transformation of microglia during normal development and in disease conditions is not clear. It is hypothesized that AMC and RMC are functionally regulated by a specific set of genes encoding various signaling molecules and transcription factors. RESULTS: To address this, we carried out cDNA microarray analysis using lectin-labeled AMC and RMC isolated from frozen tissue sections of the corpus callosum of 5-day and 4-week old rat brain respectively, by laser capture microdissection. The global gene expression profiles of both microglial phenotypes were compared and the differentially expressed genes in AMC and RMC were clustered based on their functional annotations. This genome wide comparative analysis identified genes that are specific to AMC and RMC. CONCLUSIONS: The novel and specific molecules identified from the trancriptome explains the quiescent state functioning of microglia in its two distinct morphological states. 
ISSN : 1471-2202 
Journal Name : BMC neuroscience 
Volume : 13 
Issue Number : 1 
Publishing Year : 1433 AH
2012 AD 
Article Type : Article 
Added Date : Wednesday, March 9, 2016 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
Rangarajan ParakalanParakalan, Rangarajan Investigator  
Boran JiangJiang, Boran Researcher  
Baby NimmiNimmi, Baby Researcher  
Manivannan JananiJanani, Manivannan Researcher  
Manikandan JayapalJayapal, Manikandan Researcher  
Jia LuLu, Jia Researcher  
Samuel SW TayTay, Samuel SW Researcher  
Eng-Ang LingLing, Eng-Ang Researcher  
S Thameem DheenDheen, S Thameem Researcher  

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