Document Details

Document Type : Article In Journal 
Document Title :
Understanding gas phase modifier interactions in rapid analysis by differential mobility-tandem mass spectrometry
Understanding gas phase modifier interactions in rapid analysis by differential mobility-tandem mass spectrometry
 
Document Language : English 
Abstract : A systematic study involving the use and optimization of gas-phase modifiers in quantitative differential mobility-mass spectrometry (DMS-MS) analysis is presented using nucleoside-adduct biomarkers of DNA damage as an important reference point for analysis in complex matrices. Commonly used polar protic and polar aprotic modifiers have been screened for use against two deoxyguanosine adducts of DNA: N-(deoxyguanosin-8-yl)-4-aminobiphenyl (dG-C8-4-ABP) and N-(deoxyguanosin-8-y1)-2-amino-l-methyl-6-phenylimidazo[4,5-b]pyridine (dG-C8-PhIP). Particular attention was paid to compensation voltage (CoV) shifts, peak shapes, and product ion signal intensities while optimizing the DMS-MS conditions. The optimized parameters were then applied to rapid quantitation of the DNA adducts in calf thymus DNA. After a protein precipitation step, adduct levels corresponding to less than one modification in 10(6) normal DNA bases were detected using the DMS-MS platform. Based on DMS fundamentals and ab initio thermochemical results, we interpret the complexity of DMS modifier responses in terms of thermal activation and the development of solvent shells. At very high bulk gas temperature, modifier dipole moment may be the most important factor in cluster formation and cluster geometry, but at lower temperatures, multi-neutral clusters are important and less predictable. This work provides a useful protocol for targeted DNA adduct quantitation and a basis for future work on DMS modifier effects. 
ISSN : 1044-0305 
Journal Name : Journal of The American Society for Mass Spectrometry 
Volume : 25 
Issue Number : 7 
Publishing Year : 1435 AH
2014 AD 
Article Type : Article 
Added Date : Tuesday, March 8, 2016 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
Amol KafleKafle, Amol Investigator  
Stephen L CoyCoy, Stephen L Researcher  
Bryan M WongWong, Bryan M Researcher  
Albert J Fornace JrFornace Jr, Albert J Researcher  
James J GlickGlick, James J Researcher  
Paul VourosVouros, Paul Researcher  

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