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Document Type |
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Article In Journal |
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Document Title |
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Gamma tocotrienol, a potent radioprotector, preferentially upregulates expression of anti-apoptotic genes to promote intestinal cell survival Gamma tocotrienol, a potent radioprotector, preferentially upregulates expression of anti-apoptotic genes to promote intestinal cell survival |
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Document Language |
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English |
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Abstract |
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Gamma tocotrienol (GT3) has been reported as a potent ameliorator of radiation-induced gastrointestinal (GI) toxicity when administered prophylactically. This study aimed to evaluate the role of GT3 mediated pro- and anti-apoptotic gene regulation in protecting mice from radiation-induced GI damage. Male 10- to 12-weeks-old CD2F1 mice were administered with a single dose of 200 mg/kg of GT3 or equal volume of vehicle (5% Tween-80) 24 h before exposure to 11 Gy of whole-body γ-radiation. Mouse jejunum was surgically removed 4 and 24h after radiation exposure, and was used for PCR array, histology, immunohistochemistry, and immunoblot analysis. Results were compared among vehicle pre-treated no radiation, vehicle pre-treated irradiated, and GT3 pre-treated irradiated groups. GT3 pretreated irradiated groups, both 4h and 24h after radiation, showed greater upregulation of anti-apoptotic gene expression than vehicle pretreated irradiated groups. TUNEL staining and intestinal crypt analysis showed protection of jejunum after GT3 pre-treatment and immunoblot results were supportive of PCR data. Our study demonstrated that GT3-mediated protection of intestinal cells from a GI-toxic dose of radiation occurred via upregulation of antiapoptotic and downregulation of pro-apoptotic factors, both at the transcript as well as at the protein levels. |
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ISSN |
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0278-6915 |
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Journal Name |
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Food and chemical toxicology |
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Volume |
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60 |
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Issue Number |
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1 |
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Publishing Year |
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1434 AH
2013 AD |
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Article Type |
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Article |
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Added Date |
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Tuesday, March 8, 2016 |
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Researchers
| Shubhankar Suman | Suman, Shubhankar | Investigator | | |
| Kamal Datta | Datta, Kamal | Researcher | | |
| Kushal Chakraborty | Chakraborty, Kushal | Researcher | | |
| Shilpa S Kulkarni | Kulkarni, Shilpa S | Researcher | | |
| Kathryn Doiron | Doiron, Kathryn | Researcher | | |
| Albert J Fornace | Fornace, Albert J | Researcher | | |
| K Sree Kumar | Kumar, K Sree | Researcher | | |
| Martin Hauer-Jensen | Hauer-Jensen, Martin | Investigator | | |
| Sanchita P Ghosh | Ghosh, Sanchita P | Researcher | | |
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