Document Details

Document Type : Article In Journal 
Document Title :
The Application of Multi-Colour Fluorescence In-situHybridisation to the Analysis of Abnormal Chromosomes inNon-Hodgkin's lymphomas.
The Application of Multi-Colour Fluorescence In-situHybridisation to the Analysis of Abnormal Chromosomes inNon-Hodgkin's lymphomas.
 
Document Language : English 
Abstract : Numerical and structural chromosome aberrations are recognised as important diagnostic and prognostic indicators in human diseases, including non-Hodgkin's lymphomas (NHL). Recently, fluorescence insitu hybridization (FISH) has been used with increasing success to identify and characterise many chromosomal aberrations, leading to the development of multiple chromosome painting methods for simultaneous dctection of multiple probes in metaphase target cells. In this study, we have analysed 15 NHL biopsy samples and 5 human lymphoma cell lines (Daudi. HS602, Raji, MC116 and Namalwa). Some of the cell liiics were partially analysed cytogenetically in the early 1970s using solid staining techniques, but no chromosome analysis has been previously attempted on the HS602 cell line. The detection of chromosome abnoimalities in malignant lymphoma patients and cell lines by conventional cytogenetics is particularly problematic because of difficulty in routineIy preparing metaphases spreads of sufficient quality or quantity and the complex nature of many of chromosomal changes. We completed cytogenetic analyses on all of the NHL samples and the cell lines. We then followed a strategy of refining these preliminary findings by applying multicolour FISH with whole chromosome painting probes derived from flow sorted chromosome libraries, using direct and indirect combinatorial labelling with two fluorochromes, to detect up to five different chromosomes on the same target metaphase. The application of multiple chromosome painting has enabled complex rearrangements to be. characterised and made the identification of marker chromosomes possible. 
ISSN : 1096-9896 
Journal Name : Journal of Pathology 
Volume : 182 
Issue Number : 1 
Publishing Year : 1418 AH
1997 AD 
Article Type : Article 
Added Date : Monday, May 3, 2010 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
محمد حسين القحطانيAl-Qahtani, Mohammed HussainInvestigatorDoctoratemhalqahtani@kau.edu.sa

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