Document Details

Document Type : Article In Journal 
Document Title :
Association of Specific Structural and Numerical Chromosome Abnormalities in Lymphoma Cell Lines: The Extent of Genetic Instability
Association of Specific Structural and Numerical Chromosome Abnormalities in Lymphoma Cell Lines: The Extent of Genetic Instability
 
Document Language : English 
Abstract : To carry out karyotopic analysis of lymphoma cells and on their in-vitro passages to ascertain the extent of genetic instability with an aim to understand the genetic etiology of cancer in general. Human malignant cell lines obtained from ECACC were incubated and cultured. Cytogenetic slides were prepared as per standard protocol. G-banding was carried out description of chromosomal rearrangements followed the norms of ISCN. Five lymphoma cell lines (Daudi, Raji, MC116, HS602, Namalwa) were analyzed to determine the extent of structural and numerical variation using Giemsa band analysis. All cell lines displayed abnormal chromosomes. The aneusomics observed were, in general, consistent with the aneusomics determined by previous analyses with some added complexicities. All cell lines displayed a range of aneusomies involving trisomies of chromosome number 7 and 18, while some chromosomes were not involved in aberrations at all. MC116 and Namalwa cell lines were prone to most of the structural abnormalities. Except amalwa, which was found to be consistently hypodipliod, others were hyperdiploid. High levels of karyotype heterogeneity and marker were found. Analysis showed a wide range of numerical changes affecting multiple chromosomes in lymphoma cell lines. The data suggest that chromosomal instability (CIB) is responsible for extensive aneuploidy associated with these tumors. Cytogenetic characterizations of the five human lymphoma cell lines were attempted. The study revealed new information since their deposition with ATCC. The karyotypic differences in cells from different clones and the degree of heterogeneity in the cell population observed is an indication of the involvement of different chromosome regions, with the possible implication at the level of gene expression and the structure of genes itself. The study is expected to help identify characteristic chromosome abnormalities in human lymphoma cell lines. 
ISSN : 1995-8943 
Journal Name : Academic Journal of Cancer Research 
Volume : 2 
Issue Number : 1 
Publishing Year : 1430 AH
2009 AD 
Article Type : Article 
Added Date : Wednesday, April 14, 2010 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
عادل محمد أبو زنادةAbuzenadah, Adel MohammedInvestigatorDoctorateaabuzenadah@kau.edu.sa

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