Document Details

Document Type : Article In Journal 
Document Title :
Flanking Variation Influences Rates of Stutter in Simple Repeats
Flanking Variation Influences Rates of Stutter in Simple Repeats
 
Document Language : English 
Abstract : It has been posited that the longest uninterrupted stretch (LUS) of tandem repeats, as defined by the number of exactly matching repeating motif units, is a better predictor of rates of stutter than the parental allele length (PAL). While there are cases where this hypothesis is likely correct, such as the 9.3 allele in the TH01 locus, there can be situations where it may not apply as well. For example, the PAL may capture flanking indel variations while remaining insensitive to polymorphisms in the repeat, and these haplotypic changes may impact the stutter rate. To address this, rates of stutter were contrasted against the LUS as well as the PAL on different flanking haplotypic backgrounds. This study shows that rates of stutter can vary substantially depending on the flanking haplotype, and while there are cases where the LUS is a better predictor of stutter than the PAL, examples to the contrary are apparent in commonly assayed forensic markers. Further, flanking variation that is 7 bp from the repeat region can impact rates of stutter. These findings suggest that non-proximal effects, such as DNA secondary structure, may be impacting the rates of stutter in common forensic short tandem repeat markers. 
ISSN : 2073-4425 
Journal Name : Genes 
Volume : 8 
Issue Number : 11 
Publishing Year : 1438 AH
2017 AD 
Article Type : Article 
Added Date : Wednesday, June 26, 2019 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
August E. WoernerWoerner, August E.InvestigatorDoctorateAugust.Woerner@unthsc.edu
Jonathan L. KingKing, Jonathan L.ResearcherDoctorate 
Bruce BudowleBudowle, Bruce InvestigatorDoctorateBruce.Budowle@unthsc.edu

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