Document Details

Document Type : Article In Journal 
Document Title :
A Comparison and Integration of MiSeq and MinION Platforms for Sequencing Single Source and Mixed Mitochondrial Genomes
A Comparison and Integration of MiSeq and MinION Platforms for Sequencing Single Source and Mixed Mitochondrial Genomes
 
Document Language : English 
Abstract : Single source and multiple donor (mixed) samples of human mitochondrial DNA were analyzed and compared using the MinION and the MiSeq platforms. A generalized variant detection strategy was employed to provide a cursory framework for evaluating the reliability and accuracy of mitochondrial sequences produced by the MinION. The feasibility of longread phasing was investigated to establish its efficacy in quantitatively distinguishing and deconvolving individuals in a mixture. Finally, a proof-of-concept was demonstrated by integrating both platforms in a hybrid assembly that leverages solely mixture data to accurately reconstruct full mitochondrial genomes. 
ISSN : 1932-6203 
Journal Name : PloS one 
Volume : 11 
Issue Number : 12 
Publishing Year : 1437 AH
2016 AD 
Article Type : Article 
Added Date : Tuesday, July 18, 2017 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
Michael R. LindbergLindberg, Michael R.ResearcherDoctorate 
Sarah E SchmedesSchmedes, Sarah EResearcherDoctorate 
F Curtis HewittHewitt, F CurtisResearcherDoctorate 
Jamie L. HaasHaas, Jamie L.ResearcherDoctorate 
Krista L. TernusTernus, Krista LResearcherDoctorate 
Dana R. KadavyKadavy, Dana RResearcherDoctorate 
Bruce BudowleBudowle, Bruce ResearcherDoctorate 

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