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Center of Excellence In Genomic Medicine Research
Document Details
Document Type
:
Article In Journal
Document Title
:
Elucidating the binding efficacy of β-galactosidase on graphene by docking approach and its potential application in galacto-oligosaccharide production
Elucidating the binding efficacy of β-galactosidase on graphene by docking approach and its potential application in galacto-oligosaccharide production
Document Language
:
English
Abstract
:
Herein, we propose the synthesis and characterization of graphene for the immobilization of b-galactosidase for improved galacto-oligosaccharide (GOS) production. The size of synthesized graphene was observed to be 25 nm by TEM analysis while interaction of enzyme with the nanosupport was observed by FTIR spectroscopy. Docking was obtained using molecular docking program Dock v.6.5 while the visual analyses and illustration of protein–ligand complex were investigated by utilizing chimera v.1.6.2 and PyMOL v.1.3 softwares. Immobilized b-galactosidase (IbG) showed improved stability against various physical and chemical denaturants. Km of IbG was increased to 6.41 mM as compared to 2.38 mM of soluble enzyme without bringing significant change in Vmax value. Maximum GOS content also registered an increase in lactose conversion. The maximum GOS production was achieved by immobilized enzyme at specific temperature and time. Hence, the developed nanosupport can be further exploited for developing a biosensor involving b-galactosidase or for immobilization of other industrially/therapeutically important enzymes.
ISSN
:
1615-7591
Journal Name
:
Bioprocess and biosystems engineering
Volume
:
39
Issue Number
:
5
Publishing Year
:
1437 AH
2016 AD
Article Type
:
Article
Added Date
:
Tuesday, July 11, 2017
Researchers
Researcher Name (Arabic)
Researcher Name (English)
Researcher Type
Dr Grade
Email
Rukhsana Satar
Satar, Rukhsana
Researcher
Doctorate
Syed Ahmed Ismail
Ismail, Syed Ahmed
Researcher
Doctorate
Mohd Rehan
Rehan, Mohd
Researcher
Doctorate
Shakeel Ahmed Ansari
Ansari, Shakeel Ahmed
Researcher
Doctorate
Files
File Name
Type
Description
41946.pdf
pdf
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