Document Details

Document Type : Article In Journal 
Document Title :
Modified DOP-PCR for improved STR typing of degraded DNA from human skeletal remains and bloodstains
Modified DOP-PCR for improved STR typing of degraded DNA from human skeletal remains and bloodstains
 
Document Language : English 
Abstract : Forensic and ancient DNA samples often are damaged and in limited quantity as a result of exposure to harsh environments and the passage of time. Several strategies have been proposed to address the challenges posed by degraded and low copy templates, including a PCR based whole genome amplification method called degenerate oligonucleotide-primed PCR (DOP-PCR). This study assessed the efficacy of four modified versions of the original DOP-PCR primer that retain at least a portion of the 50 defined sequence and alter the number of bases on the 30 end. The use of each of the four modified primers resulted in improved STR profiles from environmentally-damaged bloodstains, contemporary human skeletal remains, American Civil War era bone samples, and skeletal remains of WWII soldiers over those obtained by previously described DOP-PCR methods and routine STR typing. Additionally, the modified DOP-PCR procedure allows for a larger volume of DNA extract to be used, reducing the need to concentrate the sample and thus mitigating the effects of concurrent concentration of inhibitors. 
ISSN : 1344-6223 
Journal Name : Legal Medicine 
Volume : 18 
Issue Number : 1 
Publishing Year : 1437 AH
2016 AD 
Article Type : Article 
Added Date : Tuesday, July 11, 2017 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
Angie AmbersAmbers, Angie ResearcherDoctorate 
Meredith TurnboughTurnbough, Meredith ResearcherDoctorate 
Robert BenjaminBenjamin, Robert ResearcherDoctorate 
Harrell Gill-KingGill-King, Harrell ResearcherDoctorate 
Jonathan KingKing, Jonathan ResearcherDoctorate 
Antti SajantilaSajantila, Antti ResearcherDoctorate 
Bruce BudowleBudowle, Bruce ResearcherDoctorate 

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