Document Details

Document Type : Article In Journal 
Document Title :
BAD, a Proapoptotic Protein, Escapes ERK/RSK Phosphorylation in Deguelin and siRNA-Treated HeLa Cells
BAD, a Proapoptotic Protein, Escapes ERK/RSK Phosphorylation in Deguelin and siRNA-Treated HeLa Cells
 
Document Language : English 
Abstract : This study has been undertaken to explore the therapeutic effects of deguelin and specific siRNAs in HeLa cells. The data provided clearly show the silencing of ERK 1/2 with siRNAs and inhibition of ERK1/2 with deguelin treatment in HeLa cells. Additionally, we are providing information that deguelin binds directly to anti-apoptotic Bcl-2, Bcl-xl and Mcl-1 in the hydrophobic grooves, thereby releasing BAD and BAX from dimerization with these proteins. This results in increased apoptotic activity through the intrinsic pathway involved in rupture of mitochondrial membrane and release of cytochrome C. Evidence for inhibition of ERK1/2 by deguelin and escape of BAD phosphorylation at serine 112 through ERK/RSK pathway has been further fortified by obtaining similar results by silencing ERK 1/2 each with specific siRNAs. Increase in BAD after treatment with deguelin or siRNAs has been interpreted to mean that deguelin acts through several alternative pathways and therefore can be used as effective therapeutic agent. 
ISSN : 1932-6203 
Journal Name : PloS one 
Volume : 11 
Issue Number : 1 
Publishing Year : 1437 AH
2016 AD 
Article Type : Article 
Added Date : Monday, May 2, 2016 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
Samra HafeezHafeez, Samra Investigator  
Mahwish UroojUrooj, Mahwish Researcher  
Shamiala SaleemSaleem, Shamiala Researcher  
Zeeshan GillaniGillani, Zeeshan Researcher  
Sumaira ShaheenShaheen, Sumaira Researcher  
Mahmood Husain QaziQazi, Mahmood HusainResearcher  
Muhammad Imran NaseerNaseer, Muhammad ImranResearcher  
Zafar IqbalIqbal, Zafar Researcher  
Shakeel Ahmed AnsariAnsari, Shakeel AhmedResearcher  
Absarul HaqueHaque, Absarul Researcher  
Muhammad AsifAsif, Muhammad Researcher  
Manzoor Ahmad MirMir, Manzoor AhmadResearcher  
Ashraf AliAli, Ashraf Researcher  
Peter Natesan PushparajPushparaj, Peter NatesanResearcher  
Mohammad Sarwar JamalJamal, Mohammad SarwarResearcher  
Mahmood RasoolRasool, Mahmood Researcher  

Files

File NameTypeDescription
 38794.pdf pdf 

Back To Researches Page