Document Details

Document Type : Article In Journal 
Document Title :
Massively parallel sequencing of forensically relevant single nucleotide polymorphisms using TruSeq™ forensic amplicon
Massively parallel sequencing of forensically relevant single nucleotide polymorphisms using TruSeq™ forensic amplicon
 
Document Language : English 
Abstract : The TruSeq™ Forensic Amplicon library preparation protocol, originally designed to attach sequencing adapters to chromatin-bound DNA for chromatin immunoprecipitation sequencing (TruSeq™ ChIP-Seq), was used here to attach adapters directly to amplicons containing markers of forensic interest. In this study, the TruSeq™ Forensic Amplicon library preparation protocol was used to detect 160 single nucleotide polymorphisms (SNPs), including human identification SNPs (iSNPs), ancestry, and phenotypic SNPs (apSNPs) in 12 reference samples. Results were compared with those generated by a second laboratory using the same technique, as well as to those generated by whole genome sequencing (WGS). The genotype calls made using the TruSeq™ Forensic Amplicon library preparation protocol were highly concordant. The protocol described herein represents an effective and relatively sensitive means of preparing amplified nuclear DNA for massively parallel sequencing (MPS). 
ISSN : 1437-1596 
Journal Name : Int J Legal Med 
Volume : 129 
Issue Number : 1 
Publishing Year : 1436 AH
2015 AD 
Article Type : Article 
Added Date : Sunday, May 1, 2016 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
David H. WarshauerWarshauer, David H.Investigator  
Carey P. DavisDavis, Carey P.Researcher  
Cydne HoltHolt, Cydne Researcher  
Yonmee HanHan, Yonmee Researcher  
Paulina WalichiewiczWalichiewicz, Paulina Researcher  
Tom RichardsonRichardson, Tom Researcher  
Kathryn StephensStephens, Kathryn Researcher  
Anne JagerJager, Anne Researcher  
Jonathan KingKing, Jonathan Researcher  
Bruce BudowleBudowle, Bruce Researcher bbudowle@hsc.unt.edu

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