Document Details

Document Type : Article In Journal 
Document Title :
Metabolomic profiling of urine samples from mice exposed to protons reveals radiation quality and dose specific differences.
Metabolomic profiling of urine samples from mice exposed to protons reveals radiation quality and dose specific differences.
 
Document Language : English 
Abstract : As space travel is expanding to include private tourism and travel beyond low-Earth orbit, so is the risk of exposure to space radiation. Galactic cosmic rays and solar particle events have the potential to expose space travelers to significant doses of radiation that can lead to increased cancer risk and other adverse health consequences. Metabolomics has the potential to assess an individual's risk by exploring the metabolic perturbations in a biofluid or tissue. In this study, C57BL/6 mice were exposed to 0.5 and 2 Gy of 1 GeV/nucleon of protons and the levels of metabolites were evaluated in urine at 4 h after radiation exposure through liquid chromatography coupled to time-of-flight mass spectrometry. Significant differences were identified in metabolites that map to the tricarboxylic acid (TCA) cycle and fatty acid metabolism, suggesting that energy metabolism is severely impacted after exposure to protons. Additionally, various pathways of amino acid metabolism (tryptophan, tyrosine, arginine and proline and phenylalanine) were affected with potential implications for DNA damage repair and cognitive impairment. Finally, presence of products of purine and pyrimidine metabolism points to direct DNA damage or increased apoptosis. Comparison of these metabolomic data to previously published data from our laboratory with gamma radiation strongly suggests a more pronounced effect on metabolism with protons. This is the first metabolomics study with space radiation in an easily accessible biofluid such as urine that further investigates and exemplifies the biological differences at early time points after exposure to different radiation qualities. 
ISSN : 0033-7587 
Journal Name : Radiation research 
Volume : 183 
Issue Number : 4 
Publishing Year : 1436 AH
2015 AD 
Article Type : Article 
Added Date : Sunday, April 24, 2016 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
Evagelia C. LaiakisLaiakis, Evagelia C.Investigator  
Daniela TraniTrani, Daniela Researcher  
Bo-Hyun MoonMoon, Bo-Hyun Researcher  
Steven J. StrawnStrawn, Steven J.Researcher  
Albert J. Fornace JrFornace Jr, Albert J.Researcher  

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