Document Details

Document Type : Article In Journal 
Document Title :
Sprouty2 mediated tuning of signalling is essential for somite myogenesis
Sprouty2 mediated tuning of signalling is essential for somite myogenesis
 
Document Language : English 
Abstract : BACKGROUND: Negative regulators of signal transduction cascades play critical roles in controlling different aspects of normal embryonic development. Sprouty2 (Spry2) negatively regulates receptor tyrosine kinases (RTK) and FGF signalling and is important in differentiation, cell migration and proliferation. In vertebrate embryos, Spry2 is expressed in paraxial mesoderm and in forming somites. Expression is maintained in the myotome until late stages of somite differentiation. However, its role and mode of action during somite myogenesis is still unclear. RESULTS: Here, we analysed chick Spry2 expression and showed that it overlaps with that of myogenic regulatory factors MyoD and Mgn. Targeted mis-expression of Spry2 led to inhibition of myogenesis, whilst its C-terminal domain led to an increased number of myogenic cells by stimulating cell proliferation. CONCLUSIONS: Spry2 is expressed in somite myotomes and its expression overlaps with myogenic regulatory factors. Overexpression and dominant-negative interference showed that Spry2 plays a crucial role in regulating chick myogenesis by fine tuning of FGF signaling through a negative feedback loop. We also propose that mir-23, mir-27 and mir-128 could be part of the negative feedback loop mechanism. Our analysis is the first to shed some light on in vivo Spry2 function during chick somite myogenesis. 
ISSN : 1755-8794 
Journal Name : BMC medical genomics 
Volume : 8 
Issue Number : 1 
Publishing Year : 1436 AH
2015 AD 
Article Type : Article 
Added Date : Tuesday, April 5, 2016 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
Muhammad Abu-ElmagdAbu-Elmagd, Muhammad Investigator  
Katarzyna Goljanek WhysallWhysall, Katarzyna GoljanekResearcher  
Grant WheelerWheeler, Grant Researcher  
Andrea MünsterbergMünsterberg, Andrea Researcher  

Files

File NameTypeDescription
 38444.pdf pdf 

Back To Researches Page