Document Details

Document Type : Article In Journal 
Document Title :
Phosphatidylinositol 4-phosphate 5-kinase alpha (PIPKα) regulates neuronal microtubule depolymerase kinesin, KIF2A and suppresses elongation of axon branches
Phosphatidylinositol 4-phosphate 5-kinase alpha (PIPKα) regulates neuronal microtubule depolymerase kinesin, KIF2A and suppresses elongation of axon branches
 
Document Language : English 
Abstract : Neuronal morphology is regulated by cytoskeletons. Kinesin superfamily protein 2A (KIF2A) depolymerizes microtubules (MTs) at growth cones and regulates axon pathfinding. The factors controlling KIF2A in neurite development remain totally elusive. Here, using immunoprecipitation with an antibody specific to KIF2A, we identified phosphatidylinositol 4-phosphate 5-kinase alpha (PIPKα) as a candidate membrane protein that regulates the activity of KIF2A. Yeast two-hybrid and biochemical assays demonstrated direct binding between KIF2A and PIPKα. Partial colocalization of the clusters of punctate signals for these two molecules was detected by confocal microscopy and photoactivated localization microscopy. Additionally, the MT-depolymerizing activity of KIF2A was enhanced in the presence of PIPKα in vitro and in vivo. PIPKα suppressed the elongation of axon branches in a KIF2A-dependent manner, suggesting a unique PIPK-mediated mechanism controlling MT dynamics in neuronal development. 
ISSN : 0027-8424 
Journal Name : Proceedings of the National Academy of Sciences 
Volume : 109 
Issue Number : 5 
Publishing Year : 1433 AH
2012 AD 
Article Type : Article 
Added Date : Wednesday, March 9, 2016 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
Yasuko NodaNoda, Yasuko Investigator  
Shinsuke NiwaNiwa, Shinsuke Researcher  
Noriko HommaHomma, Noriko Researcher  
Hiroyuki FukudaFukuda, Hiroyuki Researcher  
Shinobu Imajo-OhmiImajo-Ohmi, Shinobu Researcher  
Nobutaka HirokawaHirokawa, Nobutaka Researcher  

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