Document Details

Document Type : Article In Journal 
Document Title :
Membrane proteins of human fetal primitive nucleated red blood cells
Membrane proteins of human fetal primitive nucleated red blood cells
 
Document Language : English 
Abstract : In humans, primitive fetal nucleated red blood cells (FNRBCs) are thought to be as vital for embryonic life as their counterpart, adult red blood cells (adult RBCs) are in later-gestation fetuses and adults. Unlike adult RBCs, the identity and functions of FNRBC proteins are poorly understood owing to a scarcity of FNRBCs for proteomic investigations. The study aimed to investigate membrane proteins of this unique cell type. We present here, the first report on the membrane proteome of human primitive FNRBCs investigated by two-dimensional liquid chromatography coupled with mass-spectrometry (2D-LCMS/MS) and bioinformatics analysis. A total of 273 proteins were identified, of which 133 (48.7%) were membrane proteins. We compared our data with membrane proteins of adult RBCs to identify common, and unique, surface membrane proteins. Twelve plasma membrane proteins with transmembrane domains and eight proteins with transmembrane domains but without known sub-cellular location were identified as unique-to-FNRBCs. Except for the transferrin receptor, all other 19 unique-to-FNRBC membrane proteins have never been described in RBCs. Reverse-transcriptase PCR (RT-PCR) and immunocytochemistry validated the 2D-LCMS/MS data. Our findings provide potential surface antigens for separation of primitive FNRBCs from maternal blood for noninvasive prenatal diagnosis, and to understand the biology of these rare cells. 
ISSN : 1874-3919 
Journal Name : Journal of proteomics 
Volume : 75 
Issue Number : 18 
Publishing Year : 1433 AH
2012 AD 
Article Type : Article 
Added Date : Wednesday, March 9, 2016 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
Sukumar PonnusamyPonnusamy, Sukumar Investigator  
Huoming ZhangZhang, Huoming Researcher  
Priya KadamKadam, Priya Researcher  
Qingsong LinLin, Qingsong Researcher  
Teck Kwang LimLim, Teck Kwang Researcher  
Jaspal Singh SandhuSandhu, Jaspal Singh Researcher  
Narasimhan KothandaramanKothandaraman, Narasimhan Researcher  
Aniza Puteri MahyuddinMahyuddin, Aniza Puteri Researcher  
Arijit BiswasBiswas, Arijit Researcher  
Annapoorna VenkatVenkat, Annapoorna Researcher  

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