Document Details

Document Type : Article In Journal 
Document Title :
Protective function of nicotinamide against ketamine-induced apoptotic neurodegeneration in the infant rat brain
Protective function of nicotinamide against ketamine-induced apoptotic neurodegeneration in the infant rat brain
 
Document Language : English 
Abstract : During development, anesthetics activate neuroapoptosis and produce damage in the central nervous system that leads to several types of neurological disorders. A single dose of ketamine (40 mg/kg) during synaptogenesis in a 7-day-old rat brain activated the apoptotic cascade and caused extensive neuronal cell death in the forebrain. In this study, we investigated the protective effect of nicotinamide against ketamine-induced apoptotic neurodegeneration. After 4 h, neuronal cell death induced by ketamine was associated with the induction of Bax, release of cytochrome c into the cytosol, and activation of caspase-3. One single dose of 1 mg/g nicotinamide was administered to a developing rat and was found to inhibit ketamine-induced neuroapoptosis by downregulating Bax, inhibiting cytochrome c release from mitochondria into cytosol, and inhibiting the expression of activated caspase-3. TUNEL and immunohistochemical analyses showed that ketamine-induced cell death occurred through apoptosis and that it was inhibited by nicotinamide. Fluoro-Jade-B staining demonstrated an increased number of dead cells in the cortex and thalamus after ketamine treatment; treatment with nicotinamide reduced the number of dead cells in these brain regions. Our findings suggest that nicotinamide attenuated ketamine-induced neuronal cell loss in the developing rat brain and is a promising therapeutic and neuroprotective agent for the treatment of neurodevelopmental disorders. 
ISSN : 0895-8696 
Journal Name : Journal of Molecular Neuroscience 
Volume : 47 
Issue Number : 1 
Publishing Year : 1433 AH
2012 AD 
Article Type : Article 
Added Date : Tuesday, March 8, 2016 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
Najeeb UllahUllah, Najeeb Investigator  
Ikram UllahUllah, Ikram Researcher  
Hae Young LeeLee, Hae Young Researcher  
Muhammad Imran NaseerNaseer, Muhammad Imran Researcher  
Park Moon SeokSeok, Park Moon Researcher  
Jawad AhmedAhmed, Jawad Researcher  
Myeong Ok KimKim, Myeong Ok Researcher  

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