Document Details

Document Type : Article In Journal 
Document Title :
Analysis of DNA methylation in FFPE tissues using the MethyLight technology
Analysis of DNA methylation in FFPE tissues using the MethyLight technology
 
Document Language : English 
Abstract : Novel biomarkers are sought after by mining DNA extracted from formalin-fixed, paraffin-embedded (FFPE) tissues. Such tissues offer the great advantage of often having complete clinical data (including survival), as well as the tissues are amenable for laser microdissection targeting specific tissue areas. Downstream analysis of such DNA includes mutational screens and methylation profiling. Screening for mutations by sequencing requires a significant amount of DNA for PCR and cycle sequencing. This is self-inhibitory if the gene screened has a large number of exons. Profiling DNA methylation using the MethyLight technology circumvents this problem and allows for the mining of several biomarkers from DNA extracted from a single microscope slide of the tissue of interest. We describe in this chapter a detailed protocol for MethyLight and its use in the determination of CpG Island Methylator Phenotype status in FFPE colorectal cancer samples. 
ISSN : 1617790540 
Journal Name : Formalin-Fixed Paraffin-Embedded Tissues: Methods and Protocols 
Volume : 724 
Issue Number : 1 
Publishing Year : 1432 AH
2011 AD 
Article Type : Article 
Added Date : Tuesday, March 8, 2016 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
Ashraf DallolDallol, Ashraf InvestigatorDoctorate 
Waleed Al-AliAl-Ali, Waleed Researcher  
Amina Al-ShaibaniAl-Shaibani, Amina Researcher  
Fahd Al-MullaAl-Mulla, Fahd Researcher  

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