Document Details

Document Type : Article In Journal 
Document Title :
Pilot study of DNA extraction from archival unstained bone marrow slides: comparison of three rapid methods.
Pilot study of DNA extraction from archival unstained bone marrow slides: comparison of three rapid methods.
 
Document Language : English 
Abstract : DNA can be isolated from a variety of human sample sources including anti-coagulant whole blood, bloodstains, hairs, tissue samples and buccal epithelial cells. The purpose of this study was to compare yield and quality of DNA samples obtained with the use of three different methods. The ability of these procedures to provide DNA for polymerase chain reaction (PCR) amplification from archival unstained bone marrow slides was tested on 35 different patients’ slides. Boiling in distilled water (A), proteinase K/Tween 35 method coupled with simplified phenol/chloroform isoamyl alcohol protocol (B) and modified commercial nucleon extraction and purification protocol (C, Amersham Life Science) gave extraction efficiencies of 57, 74 and 100% respectively. Our results demonstrate that rough DNA extraction methods have decreased efficiencies compared to complete DNA extraction protocols and that the latter are required to ensure highly reproducible results from archival unstained bone marrow slides. 
ISSN : 1684-5315 
Journal Name : African Journal of Biotechnology 
Volume : 5 
Issue Number : 6 
Publishing Year : 1427 AH
2006 AD 
Article Type : Article 
Added Date : Saturday, April 24, 2010 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
ممدوح قاريQari, Mamdooh InvestigatorDoctoratemgari@kaau.edu.sa
عادل أبو زنادةAbuzenadah, Adel ResearcherDoctorate 
عديل شودريChaudhary, Adeel ResearcherDoctorate 
محمد القحطانيAl-Qahtani, Mohammed ResearcherDoctorate 

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