تفاصيل الوثيقة

نوع الوثيقة : مقال في مجلة دورية 
عنوان الوثيقة :
Results of a collaborative study on DNA identification of aged bone samples
Results of a collaborative study on DNA identification of aged bone samples
 
لغة الوثيقة : الانجليزية 
المستخلص : AIM: A collaborative exercise with several institutes was organized by the Forensic DNA Service (FDNAS) and the Institute of the Legal Medicine, 2nd Faculty of Medicine, Charles University in Prague, Czech Republic, with the aim to test performance of different laboratories carrying out DNA analysis of relatively old bone samples. METHODS: Eighteen laboratories participating in the collaborative exercise were asked to perform DNA typing of two samples of bone powder. Two bone samples provided by the National Museum and the Institute of Archaelogy in Prague, Czech Republic, came from archeological excavations and were estimated to be approximately 150 and 400 years old. The methods of genetic characterization including autosomal, gonosomal, and mitochondrial markers was selected solely at the discretion of the participating laboratory. RESULTS: Although the participating laboratories used different extraction and amplification strategies, concordant results were obtained from the relatively intact 150 years old bone sample. Typing was more problematic with the analysis of the 400 years old bone sample due to poorer quality. CONCLUSION: The laboratories performing identification DNA analysis of bone and teeth samples should regularly test their ability to correctly perform DNA-based identification on bone samples containing degraded DNA and potential inhibitors and demonstrate that risk of contamination is minimized. 
ردمد : 1332-8166 
اسم الدورية : Croatian medical journal 
المجلد : 58 
العدد : 3 
سنة النشر : 1438 هـ
2017 م 
نوع المقالة : مقالة علمية 
تاريخ الاضافة على الموقع : Thursday, June 27, 2019 

الباحثون

اسم الباحث (عربي)اسم الباحث (انجليزي)نوع الباحثالمرتبة العلميةالبريد الالكتروني
Daniel VanekVanek, Daniel باحث رئيسيدكتوراهdaniel.vanek@fdnas.cz
Bruce BudowleBudowle, Bruce باحثدكتوراه 
Jitka Dubska-VotrubovaDubska-Votrubova, Jitka باحثدكتوراه 
Angie AmbersAmbers, Angie باحثدكتوراه 
Jan FrolikFrolik, Jan باحثدكتوراه 
Martin PospisekPospisek, Martin باحثدكتوراه 
Ahmed Anwar Al AfeefiAl Afeefi, Ahmed Anwarباحثدكتوراه 
Khalid Ismaeil Al HosaniAl Hosani, Khalid Ismaeilباحثدكتوراه 
Marie AllenAllen, Marie باحثدكتوراه 
Khudooma Saeed Al NaimiAl Naimi, Khudooma Saeedباحثدكتوراه 

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 44545.pdf pdf 

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