تفاصيل الوثيقة

نوع الوثيقة : مقال في مجلة دورية 
عنوان الوثيقة :
Synthesis and Characterization of Cefotaxime Conjugated Gold Nanoparticles and Their Use to Target Drug‐Resistant CTX‐M‐Producing Bacterial Pathogens
Synthesis and Characterization of Cefotaxime Conjugated Gold Nanoparticles and Their Use to Target Drug‐Resistant CTX‐M‐Producing Bacterial Pathogens
 
لغة الوثيقة : الانجليزية 
المستخلص : Multidrug-resistance due to “b lactamases having the expanded spectrum” (ESBLs) in members of Enterobacteriaceae is a matter of continued clinical concern. CTX-M is among the most common ESBLs in Enterobacteriaceae family. In the present study, a nanoformulation of cefotaxime was prepared using gold nanoparticles to combat drug-resistance in ESBL producing strains. Here, two CTX-M-15 positive cefotaxime resistant bacterial strains (i.e., one Escherichia coli and one Klebsiella pneumoniae strain) were used for testing the efficacy of “cefotaxime loaded goldnanoparticles.” Bromelain was used for both reduction and capping in the process of synthesis of gold-nanoparticles. Thereafter, cefotaxime was conjugated onto it with the help of activator 1-Ethyl-3-(3-dimethylaminopropyl)-carbodiimide. For characterization of both unconjugated and cefotaxime conjugated gold nanoparticles; UV-Visible spectroscopy, Scanning, and Transmission type Electron Microscopy methods accompanied withDynamic Light Scattering were used.We used agar diffusion method plus microbroth-dilutionmethod for the estimation of the antibacterial-activity and determination ofminimuminhibitory concentration or MIC values, respectively.MIC values of cefotaxime loaded gold nanoparticles against E. coli and K. pneumoniae were obtained as 1.009 and 2.018 mg/L, respectively. These bacterial strains were completely resistant to cefotaxime alone. These results reinforce the utility of conjugating an old unresponsive antibiotic with gold nanoparticles to restore its efficacy against otherwise resistant bacterial pathogens. 
ردمد : 1097-4644 
اسم الدورية : Journal of Cellular Biochemistry 
المجلد : 1 
العدد : 1 
سنة النشر : 1438 هـ
2017 م 
نوع المقالة : مقالة علمية 
تاريخ الاضافة على الموقع : Sunday, May 28, 2017 

الباحثون

اسم الباحث (عربي)اسم الباحث (انجليزي)نوع الباحثالمرتبة العلميةالبريد الالكتروني
Shaikh SibhghatullaSibhghatulla, Shaikh باحث رئيسيدكتوراه 
Rizvi Syed MohdSyed Mohd, Rizvi باحثدكتوراه 
Shakil ShaziShazi, Shakil باحثدكتوراه 
Hussain TalibTalib, Hussain باحثدكتوراه 
Alshammari Thamir MThamir M, Alshammari باحثدكتوراه 
Ahmad WaseemWaseem, Ahmad باحثدكتوراه 
Tabrez ShamsShams, Tabrez باحثدكتوراه 
Al‐Qahtani Mohammad HMohammad H, Al‐Qahtani باحثدكتوراه 
Abuzenadah Adel MAdel M, Abuzenadah باحثدكتوراه 

الملفات

اسم الملفالنوعالوصف
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