Document Details

Document Type : Article In Journal 
Document Title :
LOW MOLECULAR WEIGHT SEA ANEMONE GYROSTOMA HELLAYTHUS NEUROTOXINS FROM THE RED SEA
سموم شقائق النعمان البحرية قليلة الوزن الجزئى من البحر الأحمر
 
Document Language : Arabic 
Abstract : The crude aqueous ethanolic extract of the collected sea anemone samples Gyrostoma helianthus were partially evaporated followed by freeze-drying. The freeze-dried material dissolved in water showed neurotoxicity to mice after i.p. injection of I ml (20 mg/ml). Mouse bioassay was used to follow the toxicity of the crude extract of sea anemone during purification by ultrafiltration through a membrane filter with a molecular weight exclusion cut off 10, 5, 3, 1 and 0.5 K Dalton. Toxicity was detected till the final filtrate of less than 0.5 K Dalton. The toxicity level was determined in Mouse Units for each membrane filtrate. The dry weights of these filtrates were also determined. Mouse bioassay was used to follow the toxicity of the freeze-dried filtrate (less than 500 dalton) after fiactionation on Biogel P2. Two different toxic compounds were detected in the Biogel P2 fractions. An HPLC method was adopted to detect the HPLC peak responsible for toxicity in order to trace the toxic compounds in further purification steps and to determine percent purity of the isolated toxic compounds 
ISSN : 34567 
Journal Name : Journal of Egyptian society for Toxicity 
Volume : 43 
Issue Number : 1 
Publishing Year : 1420 AH
1999 AD 
Article Type : Article 
Added Date : Saturday, January 8, 2011 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
محمد نصر الدين محمد جمعةGomaa, Mohamed Nasr Eldin mohamedInvestigatorDoctoratemngomaa@gmail.com

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