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Document Type |
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Thesis |
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Document Title |
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Formulation and evaluation of naringin loaded lipid vesicles: cytotoxicity and antibacterial activity assessment صياغة وتقييم الحويصلات الدهنية المحملة بالنارينجين: تقييم السمية الخلوية والنشاط المضاد للبكتيريا |
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Subject |
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Faculty of Science |
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Document Language |
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Arabic |
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Abstract |
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Objective: Naringin (NG) is a flavonoid, shows pleotropic effects such as anticancer, antimicrobial, antidiabetic activity, to name a few. Multiple research conducted on NG but mostly on pure drug effect. Very limited work is available on the formulation of naringin and its evaluation against different disease. Considering current background, present study designed for formulation and evaluation of novel naringin loaded lipid vesicles for cytotoxicity and antibacterial efficacy.
Methodology: Three different concentrations of NG-loaded lipid vesicles formulations F1, F2 and F3 (5, 10, and 25 mg NG) was prepared by “thin film hydration process” by reported standard method. For preparation of formulations, different lipids, cholesterol, and surfactant were used. After formulations preparation and stability testing, it was evaluated for antibacterial and cytotoxic effect. Antibacterial activity was carried out against Staphylococcus aureus, Escherichia coli, and Bacillus subtilis by agar-well-diffusion method. Then formulation was tested for cytotoxic assay.
Results & recommendations: IC50 of Formulation 2 (F2) was found to be 0.21 mg/ml while Formulation 1 (F1) and Formulation 3 (F3) was 0.64 and 0.26 mg/ml respectively. F2 formulation exhibited zone of inhibition ranging from 18.8±1.6 mm to 20.1±1.5mm. F1 and F3 formulations inhibited bacterial growth less effectively (zone of inhibition ranging from 10.3±1.6 mm to 16.3±1.7 mm). From results, it can be concluded and recommended that prepared formulation F2 shows best efficacy as an antibacterial and cytotoxic agent. F2 formulation excellently inhibit the growth of different strains of bacteria and high potency in cytotoxic assay.
Keywords: Naringin; formulation development; anticancer activity; cell viability assay; antimicrobial. |
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Supervisor |
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Dr. Imran Kazmi |
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Thesis Type |
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Master Thesis |
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Publishing Year |
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1445 AH
2023 AD |
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Co-Supervisor |
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Dr. Muhammad Shahid Nadeem |
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Added Date |
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Wednesday, December 27, 2023 |
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Researchers
| فيصل العتيبي | Alotaibi, Faisal | Researcher | Master | |
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