Document Details

Document Type : Thesis 
Document Title :
DETECTION OF GUT MICROBIOME FOR PATIENTS WITH TYPE 2 DIABETES MELLITUS IN MAKKAH ALMUKRAMAH REGION
تحديد الميكروبات المعوية لمرضى السكري من النوع الثاني في منطقة مكة المكرمة
 
Subject : Faculty of Science 
Document Language : Arabic 
Abstract : The gut microbiota plays a critical role in maintaining host health. Recent studies revealed an association of dysbiosis in the intestinal microbial community and many chronic diseases, specifically T2D. This disease is considered as one of the most prevalent metabolic diseases worldwide, comprising up to 90%. Saudi Arabia became one of the top five countries for the occurrence of diabetes with 4.3 million patients. We aimed to detect microbiome signature for patients with T2D by comparing the diversity of the gut microbiome between T2D patients and healthy individuals. We collected fecal samples from 22 Saudi adults, including 11 diabetic patients and 11 healthy controls. The gut microbiota signatures of participants were examined using V3-V4 hypervariable regions of the 16S ribosomal RNA (rRNA) gene. Data analysis based on operational taxonomic units (OTUs) was conducted to describe microbiome composition and identify differences between those T2D patients and healthy controls. The results revealed that butyrate producing bacteria such as Actinobacteriota phylum and Bifidobacterium genus significantly decreased in the T2DM group compared to the control group. Thus, those refer to negative association with the risk of T2D development. While Prevotella, Sutterella and UCG-003 genera which belong to inflammatory bacteria showed a significant increase among the T2DM group, which can be considered as positive risk factors for T2D development. PICRUSt analysis revealed some differences in metabolites between the two groups. T2D patients might have higher levels of iron complex outer membrane receptor genes, which impact iron metabolism in both host and microbes. T2D patients had a decrease in the genes involved in starch and sucrose metabolism, as well as oxidative phosphorylation pathways. In this study, we proposed a new perspective on gut microbiota-glucose homeostasis communication, which may aid in understanding glucose dysregulation and insulin resistance. The future efforts are needed to understand the role of these microbes and their metabolites in health and disease. Keywords: Gut microbiome, type 2 diabetes, microbial metabolites, insulin resistance, Glucose metabolism. 
Supervisor : Prof. Dr. Ahmed Bahieldin Mohamed 
Thesis Type : Doctorate Thesis 
Publishing Year : 1445 AH
2023 AD 
Added Date : Wednesday, December 27, 2023 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
الهنوف محمد الخماشAlkhammash, Alhanouf MohammedResearcherDoctorate 

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