Document Details
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Document Type |
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Thesis |
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Document Title |
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Detection and Serotyping of DENGUE Virus in Mosquitos in Jeddah Province الكشف عن فيروس حمى الضنك في البعوض وتحديد أنماطه في محافظة جدة |
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Subject |
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Faculty of Science |
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Document Language |
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Arabic |
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Abstract |
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Dengue fever caused by the Dengue virus (DENV), has emerged as a mild, up to, lethal human infection. An estimated 400 million cases have been reported worldwide. The number is expected to be expanded during the past 10 years, putting half the globe's population's life in danger. The virus is consisted of single-stranded RNA with five serotypes and is spread by mosquitos of the Flaviviridae family; Flavivirus genus; Aedes and encodes for three structural proteins and seven non-structural ones. In the Kingdom of Saudi Arabia (KSA), serotype 2 of the dengue virus (DENV-2) emerged in 1994 and caused a major epidemic in Jeddah, KSA. First dengue outbreak was appeared in Makkah in 2004, in hospitalized patients. Major factors related to the wide and rapid distribution of the virus include increased urbanization levels, migration, global commerce, weather variation, and inadequate mosquito control. In addition, the development of pesticide resistance in mosquitos, irregular use of insecticides, and shifting climatic circumstances may all be factors in the virus's spread. Detection methods used to date include the detection of viral antigens (Ag) (virus extraction and purification, immunofluorescence test, and NS1 detection assay), serological assays (plaque reduction neutralization titers (PRNT), IgM/IgG immunological assays), and RNA detection by RT-PCR. Low sensitivity, specificity, and accessibility of the protocol represent a major problem that made researchers try to find a more amenable protocol. In terms of horizontal transmission of DENV through Aedes sp, which is the primary vector for transmission, DENV-infected mosquitos infect people with dengue and DENV passes from one human to another through this vector. Once acquired, the virus requires 5-7 days of incubation before the patient exhibits various dengue fever symptoms and uninfected mosquitoes come into contact with the infected person, feed on their blood, and become infected. |
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Supervisor |
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Dr. Sherif Edris Ahmed Edris |
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Thesis Type |
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Master Thesis |
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Publishing Year |
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1444 AH
2023 AD |
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Added Date |
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Tuesday, March 7, 2023 |
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Researchers
| محمد خالد الغامدي | Alghamdi, Mohammed Khaled | Researcher | Master | |
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