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Document Type |
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Thesis |
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Document Title |
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DETECTION OF WOMEN FOLLICULAR FLUID MICROORGANISMS AT THE TIME OF OOCYTE RETRIEVAL AND ITS IMPACT ON IN VITRO FERTILIZATION OUTCOMES الكشف عن ميكروبات السائل الجريبي للمرأة خلال عملية استرجاع البويضة وتأثير ذلك على نتائج عملية التلقيح الصناعي |
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Subject |
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Faculty of Science |
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Document Language |
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Arabic |
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Abstract |
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Infertility is a public health concern worldwide and recognized as a big issue in Saudi society. There are many causes that can contribute to female infertility such as ovulatory disorders, endometrioses, tubal factors, and pelvic inflammatory disease. In addition, many lifestyle factors such as age, obesity, smoking, and others can contributing factor to infertility. Therefore, many people choose to have in vitro fertilization (IVF) to get a child. The follicular fluid (FF) is a liquid that surrounds the ovum inside the ovary and it is one of the important sources of microbes that may affect IVF outcomes. In addition, the embryo culture medium contains antibacterial agents while some types of bacteria can be resistant to these particular antibiotics and does not routinely contain any antifungal agents. Aim: The present study investigated the microbes within 50 FF of women undergoing IVF procedure and the effect of these microbes on the IVF outcomes. In addition, determined the antimicrobial susceptibility for all microbial isolates. Methodology: All microbial isolates were identified according to the morphological features, biochemical tests, and molecular methods. In addition, determined the antimicrobial susceptibility to the microbial isolates. Results: Twelve microbial species belong to six genera were detected within 42% of FF samples and 58% did not show any microbial growth. The microbiological results of FF samples were more likely to be significantly associated with the IVF outcomes. Conclusion: The FF was not always sterile but contained a varied range of microbes that may affects the IVF outcomes and a larger sample of patients need to be studied to further examine our hypothesis. Furthermore, the identification of microbes within the FF in women with repeated failed IVF cycles may provide an opportunity to initiate antimicrobial treatment prior to the next conception. |
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Supervisor |
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Dr. Samah Noor |
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Thesis Type |
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Master Thesis |
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Publishing Year |
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1442 AH
2020 AD |
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Added Date |
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Saturday, February 6, 2021 |
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Researchers
| ابتسام حمود العصيمي | Alosaimi, Ibtisam Hamoud | Researcher | Master | |
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