Document Details
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Document Type |
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Thesis |
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Document Title |
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DIVERSITY PROFILING OF HUMAN SKIN MICROBIOME IN MAKKAH-AL-MUKKARAMAH REGION التعرف على تنوع ميكروبات جلد الإنسان في منطقة مكة المكرمة |
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Subject |
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Faculty of Science |
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Document Language |
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Arabic |
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Abstract |
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The skin microbiome diversity of healthy Saudi male and female individuals resident in Jeddah, Makkah Al-Mukarramah region, Saudi Arabia was studied. The study involved analysis of 16S rRNA of the microbiome in inner elbow of the right arm after ethical approval is issued and an informed consent form is signed by participating individuals. Swab samples of bacterial DNAs were purified and the V3-V4 region of 16S rRNA was amplified. Amplicons were shipped to Beijing Genome Institute (BGI, China) for deep sequencing and microbiomes analysis. Bioinformatics analysis indicated an average sequence length per read of 296 bp assigned to a total of 28 operational taxonomic units (OTUs) across samples. Shannon and Simpson indices of alpha diversity indicated no significant differences between male and female microbiomes. Principal Coordinate Analysis (PCoA) plot indicated that the skin microbiome diversity of the two microbiomes are separated. The rarefaction measure indicated that the maximum number of sequences reads for subsequent analysis is 73,000. Phylogenetic tree indicated the existence of four phyla: Actinobacteria, Bacteroidetes, Firmicutes and Proteobacteria. The relative abundance indicated that at the phylum level, Firmicutes was shown to be the highest, while Bacteroidetes was the lowest. At the genus level, Staphylococcus was the highest, while Enterococcus was the lowest. At the species level, Bacillus cereus was the highest, while Roseomonas mucosa was the lowest. The analysis for the highly abundant OTUs indicated a dramatic difference between sexes referring to either genera or species of which Staphylococcus (spp.1 & spp.2), Erwinia spp.1, Pseudomonas spp.1, Sphingomonas spp.1, Corynebacterium spp.2, Propionibacterium acnes, Kocuria palustris are higher in males, while Bacillus cereus, Bacillus spp.1, Erwinia spp.2, Corynebacterium (spp.1 & spp.3), Micrococcus spp.1, Pseudomonas spp.2 are lower in males. In conclusion, this study shed light on microbiomes of the skin of healthy Saudi individuals with differences between males and females. |
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Supervisor |
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Prof. Dr. Rashad Rizq Al-Hindi |
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Thesis Type |
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Master Thesis |
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Publishing Year |
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1441 AH
2020 AD |
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Added Date |
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Tuesday, June 30, 2020 |
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Researchers
| حنان حميد القطان | Alqattan, Hanan Hameed | Researcher | Master | |
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