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Document Type |
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Thesis |
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Document Title |
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DNA sequence analysis of Helicobacter pylori in Jeddah city, western Saudi Arabia تحليل الحمض النووي لتسلسل بكتيريا هيلكوباكتر بيلوري في مدينة جدة، غرب المملكة العربية السعودية |
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Subject |
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Faculty of Science |
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Document Language |
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Arabic |
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Abstract |
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Helicobacter pylori (H. pylori) are Gram-negative bacteria, which colonizes the mucous lining the human stomach. The bacteria affect 50% of the population in the world. H. pylori were classified by the World Health Organization and the International Agency for Research on Cancer as a class I carcinogen in humans. The bacteria have two major virulence factors; the cytotoxin-associated gene A (cagA) and vacuolating cytotoxin A gene (vacA). This study aimed to estimate the prevalence of H. pylori among a group of patients and to correlate this with the lifestyle and nutrition. And also, to know of the prevalent virulence factor (vacA and cagA genes) in Jeddah city by sequence analysis. This study included 60 patients (Saudi and non-Saudi) with symptoms similar to H. pylori infection. Biopsy specimens were obtained from patients in the department of endoscopy at King Fahad Hospital in Jeddah, Saudi Arabia. H. pylori were identified by using the 16s rRNA sequence. Then, the screening for specific genes in H. pylori (vacA and cagA) was done by using automated DNA sequencing analysis, and the DNA sequences were compared by BLAST and sequence alignment of the vacA nucleotides that is found in all H. pylori strains in our study group with those already reported in the GeneBank from various studies. Results indicated that H. pylori infection was detected in 13.3% while 86.7% were negative samples in our study patients. Interestingly, the vacA gene was found in 8.3% while the cagA gene was not detected in any patient. The prevalence of H. pylori in Saudi patients was more significant than non-Saudis (11.7% Saudi patients for 1.7% non-Saudi). Also, the female prevalence rate was higher than males (11.7% female versus 1.7% males), and the highest infection was between age 40-49 by 6.7%. All patients with H. pylori infection were ate fast food twice to thrice times a week, while the non-infected patients with H. pylori (negative patients) were ate fast food once a week. This study showed no statistically significant differences in age, nationality, and gender between H pylori-positive cases. In conclusion, this study revealed that the vacA gene was spread in the patients infected with H. pylori in Jeddah, Saudi Arabia while the cagA gene was not detected in any isolate. |
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Supervisor |
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Dr. Faten Ahmad Alsulaimany |
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Thesis Type |
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Master Thesis |
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Publishing Year |
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1441 AH
2020 AD |
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Added Date |
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Saturday, May 30, 2020 |
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Researchers
| أبرار أحمد المحمادي | Al-Mehmadi, Abrar Ahmad | Researcher | Master | |
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