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Document Type |
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Thesis |
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Document Title |
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Impact of Broccoli Antioxidant Activity on Liver Fibrosis Induced by Carbon Tetrachloride in Rats دراسة تأثير نشاط مضادات الأكسدة في نبات البروكلي على الفئران المصابة بتليف الكبد المستحث برابع كلوريد الكربون |
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Subject |
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Faculty of Science |
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Document Language |
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Arabic |
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Abstract |
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Liver fibrosis occurred due to the excessive deposition of extracellular matrix proteins (ECM). Oxidative stress plays a major role in the pathogenesis of liver fibrosis. The current study was designed to evaluate the impact of antioxidants in broccoli to improve the liver of rats injured with liver fibrosis induced by carbon tetrachloride (CCl4). The study included 32 healthy male albino rats weighting about (170-200gm). Rats were divided into four Groups: Group1(n=7): saved as a normal control group. Groups 2 to 4 (n=23): rats were injected by CCl4 intraperitonially at dose 1ml/kg mixed with corn oil at ratio 1:1 twice weekly for three weeks to induced fibrosis and kept the Group2 (n=8) as CCl4 group. Group3 (n=7): the rats were treated with ethanolic broccoli extract at dose 100mg/kg.b.w for 15days. Group4 (n=8): the rats were fed with ethanolic broccoli extract at dose 200mg/kg.b.w for15days. The results reported that the CCl4 group (Group2) significantly increase the Aspartate Aminotransferase (AST) and Alanine Aminotransferase (ALT) levels, decrease the body weight and liver weight, increase in Malondialdehyde (MDA) level, decrease in Superoxide dismutase (SOD) and Glutathione-S-Transferase (GST) and vitamin D level compared to the normal group. Broccoli antioxidants improved and increased the body and liver weight and decreased AST, ALT and MDA. Also, GST and Vitamin D levels increase compared to CCl4 group. Histopathological investigation of CCl4 group, it showed damage and necrosis in hepatocytes, while after the treatment, this damage became less and tend to normal liver cell. This study concluded that protective effects of broccoli antioxidants may improve against liver fibrosis. Therefore, the recommendation was to consume the broccoli within daily meals to enhance antioxidants capacity.
Key Words: Liver fibrosis, Broccoli, Vitamin D, CCl4, Rats. |
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Supervisor |
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Prof. Dr. Jalaluddin Awlia |
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Thesis Type |
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Master Thesis |
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Publishing Year |
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1441 AH
2020 AD |
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Co-Supervisor |
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Dr. Maryam Al-Ghamdi |
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Added Date |
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Tuesday, May 19, 2020 |
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Researchers
| روان عوض الصحفي | Alsahafi, Rawan Awad | Researcher | Master | |
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