|
Document Type |
: |
Thesis |
|
Document Title |
: |
Investigation of Prognostic Value of Methylation Profiles in Ovarian Cancer دراسة أهمية التعابير المثيلة في استشراف مستقبل سرطان المبيض |
|
Subject |
: |
Faculty of Science |
|
Document Language |
: |
Arabic |
|
Abstract |
: |
Ovarian cancer or as it is called, “Silent Killer” is the highest deadly cancer among women. It affects more than 3% of Saudi women through their lifetime. Its danger lies in delayed diagnosis and its symptomless progression mainly at early stages. Epigenetic silencing of some genes was associated with OC. Since there are only limited reports on epigenetic alterations in OC, it is important to analyze multiple genes to have an overall picture of the epigenetic map in OC. This study aims to study the methylation profiles in a panel of key genes including KL, RASSF1A, LEP and RARB in a group of OC patients, and to validate these findings through studying the expression patterns of both KL and LEP proteins. The methylation profiles of these genes were measured in 63 Formalin-Fixed Paraffin-Embedded tumor tissues by quantitative Polymerase Chain Reaction using MethyLight assay. After that, the protein expression of the KL and LEP genes was measured using both Tissue Microarray and immunohistochemistry techniques. Our Results showed that the KL methylation had the highest frequency(63%), followed by LEP(43%) and the lowest frequencies were for RARB and RASSF1A with(20.6%and12.7%, respectively). However, the results showed a significant association between hypermethylated genes and various clinicopathological features. Furthermore, Kaplan-Meier analysis of Disease-Specific Survival was significant only with hypermethylated KL(p=0.021). With regards to the protein expression of KL and LEP, the results were consistent with the results of methylation profiles since the two hypermethylated genes were silenced/reduced at the protein expression levels. Moreover, protein overexpression of KL and LEP were associated significantly with different clinicopathological features. In conclusion, our study suggests that the KL gene could be a novel biomarker for the prediction of bad prognosis for OC patients mainly in younger women. Thus, patients with hypermethylated promoters of KL require more watchful follow-up and integrative personalized therapeutics. |
|
Supervisor |
: |
Dr. Maryam Hasan Al-Zahrani |
|
Thesis Type |
: |
Master Thesis |
|
Publishing Year |
: |
1441 AH
2020 AD |
|
Co-Supervisor |
: |
Dr. Mourad Taher Assidi |
|
Added Date |
: |
Saturday, May 16, 2020 |
|
Researchers
| فاطمة محمد يحيى | Yahya, Fatimah Mohammed | Researcher | Master | |
|