Document Details

Document Type : Thesis 
Document Title :
MOLECULAR CHARACTERIZATION STUDY ON MICE COLON CANCER AFTER EXPOSURE TO LACTOBACILLUS RHAMNOSUS AND 5-FLUOROURACIL DRUG
دراسة الخصائص الجزيئية المتعلقة بسرطان قولون الفئران بعد التعرض لبكتيريا Lactobacillus rhamnosus وعقار 5-فلورويوراسيل
 
Subject : faculty of science 
Document Language : Arabic 
Abstract : 5-Fluorouracil (5-FU) has been introduced as the most common anticancer therapy despite that its response rate as a single agent is usually less than 20%. Lactobacillus (L) rhamnosus bacteria exhibit a great impact on the gastrointestinal tract infections and cancer prevention. This study illustrates the histological and Immunological changes associated with the treatment of L. rhamnosus combined to 5-FU on mice colon cancer. Fifty male mice were classify into 5 groups as follow; (A group) mice injected with Azoxymethane (AOM) with a weekly dose of 10mg/kg for four weeks to induce colon cancer, (AL group) mice injected with AOM and orally given L. rhamnosus alone, (AF group) mice injected with AOM and injected 5-FU, (AFL group) mice injected with AOM and treated with both L. rhamnosus and 5-FU, and finally (C group) untreated control mice. A reduction in the inflammation features and a normal histological structure was observed in the colon of AFL mice group compared with other treated mice groups, which seemed more similar to the untreated mice group. A significant downregulation in the level of Kras and T-Regulatory cell /IL10 transcriptions, associated with an improvement in the innate and the adaptive immune responses through increasing in the TLR2 and Th1/IFNγ transcription was verified in the intestine mucosa of AFL mice group. TNFα and IL6 elevated significantly in the serum of AFL mice groups compared to A and AF mice groups. This study provide evidence about the high potential immunological influence of L. rhamnosus during the usage of 5-FU as anticancer drug and illustrate the positive impact on colon cancer suppression. 
Supervisor : Dr. Sahar R.M. El Hadad 
Thesis Type : Master Thesis 
Publishing Year : 1441 AH
2019 AD 
Co-Supervisor : Dr. Alawaiah El Hebshi 
Added Date : Thursday, December 19, 2019 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
بشرى عمر الحازميAlhazmi, Boshra OmarResearcherMaster 

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