Document Details

Document Type : Thesis 
Document Title :
DETECTION OF SOME HLA (CLASS II) ALLELES IN TYPE I DIABETES IN JEDDAH, SAUDI ARABIA
الكشف عن بعض الأليلات المضادة من كريات الدم البيضاء (المجموعة الثانية) في داء السكري من النوع الأول في جدة،السعودية
 
Subject : Faculty of Science 
Document Language : Arabic 
Abstract : Type 1 diabetes (T1D) is a chronic autoimmune disorder that caused by destruction of β-cells of pancreas. Genetic and environmental factors are involved in pathogenesis of T1D. This study aims to determine the association between Human Leukocyte Antigens (HLA) Class II alleles encoding DR and DQ haplotypes and T1D in Saudi children. The genetic correlation of HLA*DRB1, DQA1, and DQB1 and T1D genetic susceptibility has been described by describing the T1D genetic susceptibility and protective conferred by HLA*DRB1, *DQA1, *DQB1 and determine the frequency among T1D. Ninety-six Saudi patients with T1D and 96 control subjects were enrolled in this study. Human Leukocyte Antigens were analyzed by polymerase chain reaction sequence specific oligonucleotide technique. Significant associations were found between T1D and DRB1*04:05 allele (p 0.001; OR = 3.14; 95% CI: 1.59-6.16), following by DRB1*04:02 allele (p 0.010; OR = 2.87; 95% CI: 1.33-6.22. While DQB1*06:03 allele (p 0.013) and DRB1*13:01 (p 0.0067) were significantly unassociated with T1D. The frequencies of the DRB1*04:05-DQA1*03:01-DQB1*03:02 haplotype (p 0.004), DRB1*04:02-DQA1*03:01-DQB1*03:02 haplotype (p 0.005), and DRB1*04:02-DQA1*03:01-DQB1*02:02 haplotype (p 0.008) were significantly higher in T1D patients. on the contrary, DRB1*13:01-DQA1*01:03-DQB1*06:03 haplotype (p 0.034; OR: 0.12; 95% CI: 0.014-0.94) was significant higher in control. There is no risk between DQA1-DQB1 haplotypes and T1D. In contrast, 30% of T1D patients had DRB1*04:02-DQA1*03:01 haplotype (p 0.005; OR: 3.03; 95% CI: 1.44-6.39). following by, DRB1*04:02-DQA1*05:01 haplotype (p 0.031; OR: 4.43; 95% CI: 1.21-16.24) and DRB1*04:05-DQA1*03:01 haplotype (p 0.006; OR: 4.43; 95% CI: 1.60-15.32). In addition, significant associated was presented in DRB1*04:05-DQA1*03:02 haplotype (p 0.006; OR: 3.47; 95% CI: 1.46-8.21). following by, DRB1*04:05-DQA1*05:01 haplotype (p 0.016; OR: 4.26; 95% CI: 1.36-13.35). Furthermore, DRB1-DQB1 haplotype revealed the highest associated haplotype to T1D which was DRB1*04:05-DQB1*03:02 (p <0.001; OR: 8:54; 95% CI: 2.85-25.60). A quarter of T1D patients (n=24) had DRB1*04:02-DQB1*03:02 haplotype comparing with a tenth of controls (p 0.022; OR: 2.71; 95% CI: 1.21-6.06). The results showed that the DRB1*04:02-DQB1*02:01 haplotype presented in 13.54% T1D patients and 4.17% controls (p 0.042, OR: 3.60; 95% CI: 1.13-11.48) are equally important. Our investigation indicated that there is a highly significant association between the studied alleles and haplotypes and T1D. Suggesting that DRB1*04:02 and DRB1*04:05 alleles and DRB1*04:05-DQA1*03:01-DQB1*03:02 and DRB1*04:02-DQA1*03:01-DQB1*03:02 haplotypes might be considered as susceptibility risk factors for developing of T1D in Saudi children, while the DQB1*06:03, DRB1*13:01 DRB1*10:01 alleles and DRB1*13:01-DQA1*01:03-DQB1*06:03 haplotype may consider as defense factors for T1D. 
Supervisor : Dr. Ola Ibrahim El-Hemshery 
Thesis Type : Master Thesis 
Publishing Year : 1441 AH
2019 AD 
Co-Supervisor : Dr. Syed Kashif Zaidi 
Added Date : Tuesday, November 12, 2019 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
مها غرم الله الغامديAlGhamdi, Maha GhormallahResearcherMaster 

Files

File NameTypeDescription
 45160.pdf pdf 

Back To Researches Page