Document Details

Document Type : Thesis 
Document Title :
Monitoring of gut microbiota of fish in polluted area in Jeddah coast
مراقبة جراثيم أمعاء الأسماك في المنطقة الملوثة في البحر الأحمر
 
Subject : Faculty of Science 
Document Language : Arabic 
Abstract : Trillions of bacteria form a symbiotic relationship with the digestive system of mammals, as well as in marine and aquatic animals, play a major role in health and disease. These bacteria are known as "gut microbiota,” their function not only to aid in digestion but also to produce certain types of vitamins to destroy toxins and to affect the immune system of the host. These functions could be affected by the host and non-host factors such as stress. The present study aimed to assess Next Generation Sequencing-based metagenomic analysis of microbial communities associated with oxidative stress in fish obtained from polluted (PO) versus unpolluted reference (CR) areas from the Red Sea coastal of Jeddah city in Saudi Arabia. Oxidative stress biomarkers were investigated, and gut microbiota was identified. The results showed that while superoxide dismutase (SOD) and catalase (CAT) levels were reduced in the PO site; significant elevation (P < 0.05) were observed at CR site. The decline in the relative abundance of specific dominant genera was observed between PO and CR sites. Diversity and bacterial richness had been observed in the PO site compared to the CR site. Metagenomic analysis revealed dominant genera, which were correlated with stress conditions. As a result, 429,346 sequences respectively for PO and CR were obtained from 20 samples identified into ten phyla and 79 genera in which Firmicutes was dominant in both PO and CR sites. The number of common OTUs (operational taxonomic units) was 221 for both CR and PO samples. The results revealed that gut microbiota was influenced by stress condition leading to shifting in oxidative stress for the survival ability in extreme conditions. Under the stressed environmental condition, homo-lactic Vagococcus genus is dominant over the hetero-lactic Lactobacillus genus. This explains the homofermentation phenomenon of the Vagococcus sp., which produces more lactic acid and energy from glucose than heterofermentation of the Lactobacillus sp. to overcome pollution-induced stress conditions. 
Supervisor : Dr. Othman A. Baothman 
Thesis Type : Master Thesis 
Publishing Year : 1440 AH
2019 AD 
Co-Supervisor : Prof. Abu-Bakr M. Gomaa 
Added Date : Tuesday, August 27, 2019 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
سالم محمد الزهرانيAlZahrani, Salem MohammedResearcherMaster 

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