Document Details
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Document Type |
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Thesis |
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Document Title |
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Relationship between Resistin and Inflammation in Saudi Females with and without Osteoarthritis Disease العلاقة بين الريزيستين والالتهاب في السيدات السعوديات المصابات وغير المصابات بمرض التهاب المفاصل العظمي |
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Subject |
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Faculty of Medicine |
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Document Language |
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Arabic |
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Abstract |
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Osteoarthritis disease is one of the most common degenerative joint diseases in aging population. Obesity is an important risk factor for initiation and progression of osteoarthritis disease. It is accepted that excess body weight may lead to cartilage degeneration by increasing the mechanical forces across weight-bearing joints. However, emerging data suggest that additional metabolic factors released mainly by white adipose tissue may also be responsible for the high prevalence of osteoarthritis disease among obese people. Adipocyte-derived molecules ‘‘adipokines’’ have prompt much interest in osteoarthritis disease pathophysiological research over the past decade since they play an important role in cartilage and bone homeostasis. Recently, among the family of adipokines, resistin was found to be relevant to inflammation and inflammation-related diseases such as osteoarthritis disease as inflammatogenic cytokine. The pro-inflammatory profile of resistin, together with its association with other factors such as obesity, aging, dietary habits suggest that this adipokine might be another potential mediator that links osteoarthritis with inflammation.
Our study hypothesis suggested that high resistin levels may play important role in osteoarthritis progression. Therefore, the aim for this study is to investigate the relationship between resistin and inflammation in Saudi females with and without osteoarthritis.
A case–control study was undertaken of 200 Saudi women (aged between 38 and 61 years) with and without osteoarthritis disease who were randomly recruited from the Orthopedic clinics at King Abdulaziz University Hospital, Jeddah, Saudi Arabia.
All study subjects were asked using questionnaire for demographic data, clinical history, lifestyle habits, level of physical activities, and dietary intake for the past 12 months. Clinical data included medical history, use of medication and co-morbidity diseases. Grading of osteoarthritic patients was estimated according to Kellgren-Lawrence classification system. Subjects with osteoarthritis were asked to determine the joints affected with disease and the type of procedures that was performed to confirm osteoarthritis diagnosis.
Anthropometric measurements were taken for all study subjects, including: body weight, height, waist and hip circumferences. Body mass index and waist hip ratio were calculated for all study population. Systolic and diastolic blood pressure measurements were taken for all study subjects.
Biochemical characteristics were measured in serum samples including: fasting blood glucose, fasting lipid profile including (total cholesterol, triglycerides, and direct high density lipoprotein, low density lipoprotein, very low density lipoprotein), high sensitivity-C reactive protein, and erythrocyte sedimentation rate. Complete blood count was measured as whole blood.
Human serum resistin levels were measured by Enzyme Linked Immuno-Sorbent Assay at King Fahad Medical Research Center, KAU.
Osteoarthritis patients had lower body mass index values (p<0.01) than their age matched controls. Hip circumference values were significantly higher among osteoarthritis group in comparison with the non-osteoarthritis group (p<0.0001), the majority of the osteoarthritic patients (91%) had their knees joints affected by osteoarthritis, and most of them (83%) were of moderate grade. About 64% of osteoarthritic individuals had moderate joint pain with going up or down the stairs, and 75% reported moderate joint pain with praying (i.e., bending to the floor and rising from sitting). Mean values of erythrocytes sedimentation rate and high sensitivity- C reactive protein were significantly higher (p <0.0001) in the patients with osteoarthritis than their matching controls.
Osteoarthritic patients reported significantly lower intake with respect to the following dietary items: energy (p<0.0001), percentage of calories provided by carbohydrates (p<0.05), cholesterol, vitamins A, C, B12 (p<0.0001), folic acid (p<0.05), iron, selenium, zinc and copper (p<0.0001). While, mean values of dietary intakes of total calories provided by protein (p<0.05), polyunsaturated fatty acids and monounsaturated fatty acids (p<0.01) were significantly higher among osteoarthritic patients than their control counterparts.
Interestingly, mean values of serum resistin levels were significantly higher among the study subjects with osteoarthritis than those without osteoarthritis (p<0.0001). Also, there are positive associations between serum resistin levels and high sensitivity- C reactive protein (mg/L) (r=0.498, p<0.0001) and with erythrocytes sedimentation rate (mm/hr) (r=0.869, p<0.0001). On the other hand, resistin was negatively correlated with mean values of body mass index (Kg/ m2) (r= - 0.175, p<0.01).
Resistin, as a novel adipokine, may represents a link between inflammation and osteoarthritis via its pro‐inflammatory pathways. Serum levels of resistin are associated with inflammatory markers (namely erythrocytes sedimentation rate and high sensitivity-C- reactive protein), suggesting that it has a role to play in the pathogenesis and inflammation of osteoarthritis disease. Furthermore, coexistence of high serum levels of resistin, erythrocytes sedimentation rate, and high sensitivity - C - reactive protein among osteoarthritis patients may be considered useful indicators in inflammatory process of osteoarthritis disease.
A clearer understanding of the relationship between resistin and inflammation with osteoarthritis would provide health professionals with significant information in terms clinical practice. Nevertheless, further studies are needed to investigate the sequence of this association. Also, further experiments are still crucial for understanding the relationship between resistin and osteoarthritis disease. |
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Supervisor |
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Prof. Eman Moqbel Alissa |
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Thesis Type |
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Master Thesis |
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Publishing Year |
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1440 AH
2019 AD |
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Added Date |
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Monday, March 4, 2019 |
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Researchers
| ليلى صالح الزغبي | AL-Zaghabi, Layla Saleh | Researcher | Master | |
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