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Document Type |
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Thesis |
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Document Title |
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Removal of some heavy metals from industrial wastewater by actinomycetes isolated from soil ازالة بعض المعادن الثقيلة من مخلفات مياه المصانع بواسطة الاكتينوميسيتات المعزولة من التربة |
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Subject |
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Faculty of Sciences |
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Document Language |
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Arabic |
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Abstract |
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This study was aimed to examine the ability of bacteria to remove heavy metals. Out of 23 isolates, recovered from different contaminated soil area from industrial wastewater Treatment plant (IWWTP), located in Jeddah, strain FM1 FM2, FM3, and FM4 were the most potent in removing of heavy metals. The 16S rRNA sequence of metal tolerant bacterial isolate FM1 showed the highest (98%) similarity with Streptomyces toxytricini and the second isolate FM2 was showed the highest (98%) similarity with Streptomyces mutabilis while the third isolate FM3 showed the highest (97%) similarity with Streptomyces lienomycini, finally the fourth isolate FM4 showed the highest (93%) similarity with Streptomyces sp. The growth of the previous isolates was determined after 5 days at 30℃ in the presence of different heavy metals concentrations, 3000- 5500 mg/l of lead acetate, and 50- 300 mg/l of chromium oxide. The most resistant isolate for lead was isolate FM1(MIC 5200 mg/l) but the most resistant isolate for Cr (VI) was FM2 (MIC135 mg/l). By dead biomass of isolate FM1, the biosorption capacity of the strain for the metallic ions was highest for Pb++, 99.95%, at 800 mg/l Pb++ while the lowest sorption by bacterial biomass was recorder at 400 mg/l. Moreover, the biosorption capacity of the dead bacterial biomass of the isolate FM1 at 100 mg/l of Cr+ ion was 38% of the initial metal ion concentration while the lowest biosorption was 21.54% at 400 mg/l. The efficiency of biosorption was tested by the metal ion concentration analyzed using Plasma Atomic Emission Spectrometer ICPE-9000. For the two tested isolates, the optimum pH for lead removal was at pH 6-8 and the optimum pH for chromium removal was pH 7. On the other hand, the optimum temperature for lead removal was 28 and 45℃ for FM1 and FM2, respectively while maximum chromium removal was at 37 and 45℃ for FM1 and FM2, respectively. Biosorbent mass of 0 - 1.5 g was tested for removal of chromium and lead. The result showed that the adsorption capacities against heavy metals, Pb++and Cr (VI), was increased with increasing the weight of the used dry biomass. The result showed that FM1 and FM2 biomass has a good potential to be used in removal of metal ions from waste water. Their use in real life situation can alleviate pollution and increase the quality of water for human consumption and sanitary purposes. |
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Supervisor |
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Prof. Dr. Magda Mohammed Aly |
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Thesis Type |
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Master Thesis |
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Publishing Year |
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1440 AH
2019 AD |
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Co-Supervisor |
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Dr. Nidal Mohammed Zabermawi |
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Added Date |
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Tuesday, February 26, 2019 |
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Researchers
| فاطمة محمد بكران | Bakran, Fatima Mohammed | Researcher | Master | |
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