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Document Type |
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Thesis |
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Document Title |
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Genotoxicity and Antigenotoxicity Interactive Activites of Rhazya stricta and Zingiber officinale النشاط التفاعلي للسمية الجينية وتضادها لنبات الحرمل والزنجبيل |
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Subject |
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Faculty of Sciences > biological sciences department |
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Document Language |
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Arabic |
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Abstract |
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Rhazya stricta is commonly used in folk medicine in the Arabian Peninsula for the treatment of many diseases such as diabetes mellitus. In many studies, relatively large doses of the plant extract were used to determine the pharmacological and toxicological actions. Therefore, it was necessary to study the biochemical effects of this plant using low doses, almost near the dose that is used by humans in the folk medicine. In the present study we will investigate the genotoxicity and antigenotoxicity of Rhazya stricta leaves and Zingiber officinale rhizomes as single and in combinations using Salmonella typhimurium mutant test and single cell gel electrophoresis comet assay at the maximum tolerant dose for each assay. These two plants are important plants and they are widely used in folk medicine. Although these plants are heavily investigated but their mixtures has not .So we intended to investigate the possible occurrence of antagonistic, additive or synergistic interactions implications for risk assessment. Result showed that none of R.stricta and Z.officinale extraction in single and in combinations showed any mutagenicity effect on the battery of Salmonella typhimurium mutant test strains TA100, TA102, TA98, TA79a and TA1535, at concentration of 10mg/plat. In comet assay Results showed genotoxicity effect of the R.stricta (1.5g/kg) and Z.officinale (10g/kg) extraction produced significant (P< 0.05) effects on comet percent and the mean tail moment value in single and combination, the result for the same dose in antigenotoxicity test by comet showed no significant result of the R.stricta and Z.officinale in single and combination (P> 0.05) via MNNG at dose 30 μg/ml of blood. R.stricta and Z.officinale extraction in single and in combinations increased the cytochrome p450 1A1 concentration in mice liver (P< 0.01). However, more work is needed, with the growing interest of the world in complementary and alternative medicines applications, to investigate possible mechanisms of action of Rhazya stricta leaves , using the same method of extraction that have been used by humans in the folk medicine |
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Supervisor |
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Prof. Sufian. M. ELassoli |
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Thesis Type |
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Master Thesis |
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Publishing Year |
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1436 AH
2014 AD |
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Co-Supervisor |
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Dr. Mohammed Hamed Zainy |
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Added Date |
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Sunday, December 7, 2014 |
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Researchers
| أمل أحمد المستادي | Almostady, Amal Ahmed | Researcher | Master | |
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